Electrochemical sensor for rapidly and selectively detecting quercetin and preparation method thereof

A quercetin and sensor technology, which is applied in the field of electrochemical sensors for rapid and selective detection of quercetin and its preparation, can solve the problem that the electrochemical detection of quercetin is rarely studied, and achieves low preparation cost and high sensitivity , the effect of easy operation

Inactive Publication Date: 2019-05-14
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrochemical detection of quercetin has rarely been studied.

Method used

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  • Electrochemical sensor for rapidly and selectively detecting quercetin and preparation method thereof
  • Electrochemical sensor for rapidly and selectively detecting quercetin and preparation method thereof
  • Electrochemical sensor for rapidly and selectively detecting quercetin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of an electrochemical sensor for fast and selective detection of quercetin, comprising the following steps:

[0029] Step 1: Preparation of Composite Materials

[0030] Place the glassy carbon electrode in 5 mL of acetonitrile solution containing 0.0808 g of anhydrous lithium perchlorate and 5 μL of EDOT for potentiostatic polymerization for 15 s, and the voltage window is 0-1.35 v;

[0031] Step 2: Preparation of Composite Electrode

[0032] Take 5 μL of 1mg / ml WS 2 The water dispersion was drop-coated on the surface of the glassy carbon electrode, and then dried at 60 °C to obtain WS 2 / PEDOT composite electrode.

[0033] For the WS prepared above 2 For the electrochemical response of quercetin / PEDOT composite electrode, see figure 1 , in the Britton-Robinson (Britton-Robinson) solution containing 10 μmol / L quercetin 0.2 mol / L, the composite electrode is 0.38 to quercetin v There is an irreversible oxidation peak, indicating that the composi...

Embodiment 2

[0040] A preparation method of an electrochemical sensor for fast and selective detection of quercetin, comprising the following steps:

[0041] Step 1: Preparation of Composite Materials

[0042] The glassy carbon electrode was placed in the acetonitrile solution of anhydrous lithium perchlorate and EDOT for potentiostatic polymerization for 10 s, and the voltage window was 0-1.35 v; the mass ratio of anhydrous lithium perchlorate and EDOT was 12:1; acetonitrile and EDOT The volume ratio is 500:1;

[0043] Step 2: Preparation of Composite Electrode

[0044] Take 1mg / ml WS 2 The water dispersion liquid is drop-coated on the surface of the polymerized glassy carbon electrode, WS 2 The dosage is 3 μL, after drying at 50℃, WS 2 / PEDOT composite electrode.

Embodiment 3

[0046] A preparation method of an electrochemical sensor for fast and selective detection of quercetin, comprising the following steps:

[0047] Step 1: Preparation of Composite Materials

[0048] The glassy carbon electrode was placed in the acetonitrile solution of anhydrous lithium perchlorate and EDOT for potentiostatic polymerization for 20 s, and the voltage window was 0-1.35 v; the mass ratio of anhydrous lithium perchlorate and EDOT was 13:1; acetonitrile and EDOT The volume ratio is 500:1;

[0049] Step 2: Preparation of Composite Electrode

[0050] Take 1mg / ml WS 2 The water dispersion liquid is drop-coated on the surface of the polymerized glassy carbon electrode, WS 2 The dosage is 10 μL, and after drying at 70°C, WS 2 / PEDOT composite electrode.

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Abstract

The invention discloses an electrochemical sensor for rapidly and selectively detecting quercetin and a preparation method thereof, and belongs to the technical field of electrochemical sensors. A composite electrode of tungsten disulfide and PEDOT can be prepared by the following steps: polymerizing PETDO on a glassy carbon electrode by an electrodeposition method; then coating an upper layer ofa polymeric material with 5[mu]L of WS2 aqueous dispersion in a dripping manner; and drying. The quercetin is subjected to electrochemical detection in a solution in which 0.2mol/L of Britton-Robinsonis taken as a supporting electrolyte. The composite electrode shows high electrocatalytic activity for the quercetin. The electrode can realize efficient, sensitive and selective detection of the quercetin, and can be successfully applied to determination of the quercetin content of lotus leaves. The prepared electrochemical sensor has the advantages of easy preparation of the electrode material,low cost, easiness and convenience in operation, rapidness, high efficiency and high sensitivity.

Description

technical field [0001] The invention belongs to the field of electrochemical sensors, and relates to an electrochemical sensor for fast and selective detection of quercetin and a preparation method thereof. Background technique [0002] Quercetin is an antioxidant. There are double bonds between the 2 and 3 positions in the molecule, and there are 2 hydroxyl groups at the 37 and 47 positions, so it can be used as a free radical acceptor produced in the oxidation process of metal chelation or oil. In terms of anti-cancer, quercetin can significantly inhibit the effect of cancer-promoting agents, inhibit the growth of malignant cells in vitro, and inhibit the DNA, RNA and protein synthesis of Ehrlich ascites cancer cells. However, excess quercetin can induce inflammation, joint pain and stiffness. Up to now, a variety of quercetin determination methods have been established, and the widely used methods are mainly chromatography, such as thin layer chromatography, gas chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
Inventor 段学民李莹莹文阳平徐景坤周卫强
Owner JIANGXI SCI & TECH NORMAL UNIV
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