Photocuring-based fast mounting medium as well as preparation method and application thereof

Abstract

The invention belongs to the field of application of biomedicines, and discloses a photocuring-based fast mounting medium as well as a preparation method and application thereof. The photocuring-basedfast mounting medium comprises the following components in percent by mass: 80-96% of a photocuring monomer, 3-15% of a photoinitiator and 0-8% of a functional aid. The photocuring-based fast mounting medium prepared according to the preparation method provided by the invention has the viscosity of 80-200 cps, has the refractive index after curing being greater than 1.52, and has higher refractive index, lower viscosity and shorter curing time than neutral resin; observation and photographing of a sample under a microscope are not affected; meanwhile, due to no need of using dimethylbenzene,damage to the body of a pathological technician or an experimenter is less.

Description

technical field [0001] The invention belongs to the application field of biomedicine, and in particular relates to a photocuring-based rapid mounting agent and a preparation method and application thereof. Background technique [0002] Mounting is an indicator that the slices (tissue, blood, and cell slides, etc.) are preserved in the cover glass and slide glass under the sealing of the mounting agent, which isolates oxygen and avoids further oxidation and color degradation. operate. Sections of specimens mounted with mounting medium are conducive to long-term preservation. [0003] The mounting agent that achieves the sealing effect often has the following characteristics: 1. The refraction property is close to that of the slide glass or the cover glass; 2. It can maintain the connection between the cover glass and the slide glass more firmly; 3. It is compatible with the specimen The dyes do not interact with each other. According to these characteristics, the mounting ...

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Problems solved by technology

2. Water-soluble mounting agents represented by glycerin gelatin and Arabic gelatin are less used at present due to their high price and shorter storage time of dyes than non-water-soluble mounting agents

[0005] There are many disadvantages in the existing mounting agent in actual use: 1. Since the viscosity of neutral gum is relatively large when used, the penetration process of the gum is slow and uneven, and when xylene with neurotoxicity is often required, commonly used xylene has potential The carcinogenic effect will affect the health of the experiment operator; 2. When the neutral gum is used to mount the slide, the time for the mountant to solidify and firmly bond the cover glass to the slide is relatively slow, often taking several hours or even a day, which affects the follow-up Further observe the progress of the results. For example, in the pathology department of the hospital, it is often necessary to wait for several hours or even a day for the neutral resin to solidify, which will affect the issuance of the pathology report and potentially delay the patient's treatment time; Compared with cover glass and slide glass, there is still a gap, which has an impact on the transparency of tissue observation, and cannot satisfy high-quality image acquisition under a microscope

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