Method for increasing germination rate of stevia rebaudiana seeds

A technology of stevia and germination rate, applied in the field of seed treatment in the field of agricultural technology, can solve the problems of restricting stevia yield, low emergence rate, low germination rate, etc., achieve no residual cost, increase germination rate, promote germination and seedling growth The effect of growth

Pending Publication Date: 2019-05-10
GANSU RES INST OF AGRI ENG TECH
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Problems solved by technology

In the production of stevia seeds, there are problems such as low germination rate, difficult e

Method used

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  • Method for increasing germination rate of stevia rebaudiana seeds
  • Method for increasing germination rate of stevia rebaudiana seeds

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 determines optimum concentration and optimal pretreatment time

[0018] Gently rub the stevia seeds with your hands to remove the pappus, remove the seeds and impurities by winnowing, select the stevia seeds with plump and healthy seeds and the same size, weigh 54 parts of 20g seeds, wash them with distilled water 4 times, Rinse well; then disinfect with 10% sodium hypochlorite solution for 10 minutes, rinse with distilled water 3 times, and filter dry.

[0019] Prepare different concentrations of salicylic acid to pretreat stevia seeds; the concentration of salicylic acid is 0, 0.03, 0.05, 0.10, 0.50, 1.00mmol / L; take 100mL of different concentrations of salicylic acid for each 3 (3 replicates ) x 3 (3 pretreatment time) portions, add the same amount of sterilized stevia seeds respectively, and soak the seeds in a constant temperature water bath at 25°C for 12, 24, and 36 hours respectively; after the soaking, rinse the seeds with distilled water for 3 tim...

Embodiment 2

[0026] The seeds of Huinong No. 3 stevia rebaudiana propagated by Gansu Agricultural Engineering Technology Research Institute were selected, 2000 grains were randomly divided into 1000 grains. Among them, 1000 seeds were mixed with an aqueous solution with a salicylic acid concentration of 0.05mmol / L; the other 1000 seeds were used as a control, soaked in clean water at a ratio of 1:10 for 24 hours at 25°C, then washed with clean water and air-dried seed. The seed germination test was carried out by Petri dish filter paper method. Each group of seeds that have been processed is sowed evenly on the filter paper in a petri dish (diameter 90mm) that has been wetted with distilled water and the water content is 60%. 100 seeds per dish are repeated 3 times and sprayed every day. 1 time of water, keep the filter paper moist, cover the Petri dish and put it in the incubator for cultivation. The culture temperature is 25°C, the humidity is 85%, the light is 12h / d, and the light int...

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Abstract

The invention discloses a method for increasing the germination rate of stevia rebaudiana seeds. The method has the following specific steps: selecting the stevia rebaudiana seeds with plump and healthy seeds in the same size for later use; disinfecting the seeds for 5-15 minutes by virtue of a 10% sodium hypochlorite solution, repeatedly flushing the seeds with distilled water, and filtering to dry; preparing salicylic acid in different concentrations, and carrying out pretreatment on the stevia rebaudiana seeds; respectively adding equivalent disinfected stevia rebaudiana seeds into at leastthree parts of equivalent salicylic acid in different concentrations so as to soak the seeds; and after the soaking of the seeds is finished, flushing the seeds for 3-5 times, and carrying out seed germination test by virtue of a culture dish filter paper method. The method disclosed by the invention has the advantages of convenience, high efficiency, no toxicity, no residue, low cost and the like, is high in economic benefit and convenient for agricultural production and has wide application prospects in the modern efficient agricultural production.

Description

technical field [0001] The invention relates to the seed treatment technology in the field of agricultural technology, in particular to a method for improving the germination rate of Stevia rebaudiana seeds by using the plant endogenous hormone salicylic acid as a plant growth regulator. Background technique [0002] Stevia (Stevia rebaudiana (Bertoni) Hemsl) is native to the subtropical region of South America, and is a perennial herb belonging to the genus Stevia in the family Asteraceae. Stevioside extracted from Stevia rebaudiana is known as "natural saccharin" and the "third sugar source" in the world. It is a natural, high-sweetness, low-calorie, new green health-care sweetener. An ideal substitute for sugar; glycosides have good anti-cancer activity and the effect of reducing blood sugar concentration in type 2 diabetes and controlling high blood pressure, and have great potential in the development of medical and health care products. At present, it is widely used i...

Claims

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Application Information

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IPC IPC(8): A01C1/00
Inventor 谢忠清李润喜韩钟英王致和李彦荣何友萍
Owner GANSU RES INST OF AGRI ENG TECH
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