Method for stirring assisted ultrasonic extraction of Bletilla striata polysaccharides

A polysaccharide and ultrasonic technology, applied in the field of biopharmaceuticals, can solve the problems of high energy consumption and low efficiency, and achieve the effect of low energy consumption and easy extraction

Inactive Publication Date: 2019-05-07
QUJING NORMAL UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The patent application uses ultrasound to crush frozen white and then uses heating-assisted ultrasonic continuous extraction of white and polysaccharide, which has high energy consumption and low efficiency.

Method used

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Embodiment Construction

[0011] A method for extracting polysaccharides from Baiji with stirring-assisted ultrasound, comprising the following steps.

[0012] Step 1: After washing, slicing and drying fresh bletilla tubers, they are pulverized by a pulverizer to obtain bletilla powder with a particle size of less than 80 meshes, which are sealed and stored for later use.

[0013] Step 2. By weight, take 1 part of white powder prepared in step 1 and put it into a blender, add 150 parts of drinking water into the blender, start ultrasonication and stir for 40 min at the same time to obtain a mixed material liquid. Ultrasonic frequency is 53kHz, ultrasonic power is 2800W / m 2 , The speed of the mixer is 300 r / min.

[0014] Step 3 Centrifuge the mixed material liquid in step 2 to obtain an extract, and the centrifugation speed is 4000r / min. Gradually add ethanol to the extract, so that the concentration of ethanol in the extract reaches 80%, and the polysaccharide is precipitated.

[0015] The above met...

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Abstract

The invention belongs to the technical field of biopharmaceutics, and concretely relates to a method for stirring assisted ultrasonic extraction of Bletilla striata polysaccharides. The method includes the following steps: 1, washing, slicing and drying fresh Bletilla striata tubers, crushing the Bletilla striata tubers by a crusher to obtain Bletilla striata powder, and performing sealing storageon the Bletilla striata powder for later use; 2, taking 1 part by weight of the Bletilla striata powder prepared in step 1, adding the Bletilla striata powder into a stirrer, adding 140-160 parts byweight of water into the stirrer, and simultaneously carrying out ultrasonic treatment and stirring for 35-45 min to obtain a mixed feed liquid, wherein the ultrasonic frequency is 53 kHz, the ultrasonic power is 2500-3000 W / m<2>, and the rotating speed of the stirrer is 250-350 r / min; and 3, centrifuging the mixed liquid obtained in step 2 to obtain an extract, and carrying out alcohol precipitation on the extract obtained by separation in order to obtain the polysaccharides. The method for stirring assisted ultrasonic intermittent extraction of Bletilla striata polysaccharides has the advantages of simplicity in extraction, fastness, high efficiency and low energy consumption.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a method for extracting white and polysaccharides by using stirring-assisted ultrasonic waves. Background technique [0002] Baiji is a traditional Chinese medicine, which is used as a tuber. It is rich in starch, mucus, phenanthrene derivatives, anthraquinone derivatives and other biological components, of which mucilage accounts for 40% to 50%. The main component of mucus is polysaccharide, which is a natural polymer material that has the functions of hemostasis, protection of wounds and mucous membranes of the digestive tract, antibacterial, anti-cancer, whitening, and moisturizing. Baiji is used in the treatment of clinical lung and stomach bleeding, traumatic bleeding, chapped skin, water and fire burns, and mouth and nose diseases; it is also used in the fields of drug preparation, adhesives, protective agents, cosmetics, and food. [0003] Polysacch...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杨晓丽
Owner QUJING NORMAL UNIV
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