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Brevibacterium frigoritolerans strain capable of denitrification and efficient flocculation and application thereof

A cold-resistant Brevibacterium and flocculation technology, applied in the field of microorganisms, can solve problems such as poor effect, and achieve the effect of large application potential, strong flocculation ability and good removal effect.

Active Publication Date: 2019-05-03
GUANGDONG BOWOTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of the actual water environment, direct release of free bacterial strains to water bodies for denitrification is often ineffective

Method used

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  • Brevibacterium frigoritolerans strain capable of denitrification and efficient flocculation and application thereof
  • Brevibacterium frigoritolerans strain capable of denitrification and efficient flocculation and application thereof
  • Brevibacterium frigoritolerans strain capable of denitrification and efficient flocculation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Enrichment culture, isolation, purification and preservation of Brevibacterium cold-tolerant ZQ 1-1

[0023] (1) Enrichment culture

[0024] Enrichment medium: NH 4 Cl 0.4g / L, NaNO 2 0.25g / L, KH 2 PO 4 2.0g / L, MgSO 4 ·7H 2 O 0.2g / L, Na 2 CO 3 0.4g / L, the solvent is water; sterilize at 121℃ for 20min under high temperature and high pressure. Pack into 250mL Erlenmeyer flasks, 90mL per bottle.

[0025] Take 10mL of sewage from the natural aeration tank of pig manure, put it into the Erlenmeyer flask filled with the above-mentioned culture medium, culture at 30°C and 180rpm for 48h, and carry out the enrichment of denitrification bacteria. Then transfer 10 mL to a new Erlenmeyer flask containing the above-mentioned enrichment medium, culture at 30° C., 180 rpm, for 48 hours, and perform secondary enrichment to obtain a secondary enrichment culture.

[0026] (2) Isolation, purification and preservation of Brevibacterium cold-tolerant ZQ 1-1

[0027] ...

Embodiment 2

[0029] Example 2 Identification of 16S rDNA of Brevibacterium cold-tolerant ZQ 1-1

[0030] Genomic DNA of Brevibacterium cold-resistant ZQ 1-1 was extracted, and the PCR product was amplified with bacterial 16S rDNA gene amplification general primer 27F / 1492R (5'-AGAGTTTGATCCTGGCTCAG-3' and 5'-TACGACTTAACCCCAATCGC-3'), and sent to Shanghai Meiji Biomedical Technology Co., Ltd. (Guangzhou Branch) performed sequence sequencing, and the sequence is shown in SEQ ID NO.1. The sequencing results were compared with the 16S rDNA sequences in NCBI and EzBioCloud databases for homology analysis, and the results showed that it had the highest similarity (99.48%) with the 16S rDNA gene sequence of the strain Brevibacterium frigoritolerans. Based on the analysis of the above results, the Brevibacterium frigoritolerans of the present invention was preliminarily identified as Brevibacterium frigoritolerans, and named: Brevibacterium frigoritolerans ZQ 1-1. The strain was deposited in Guang...

Embodiment 3

[0031] Example 3 Detection of Flocculation Efficiency of Brevibacterium cold-tolerant ZQ 1-1

[0032] The flocculation efficiency is detected by the kaolin suspension detection method (Kaolin method):

[0033] Preparation of Brevibacterium cold-tolerant ZQ1-1 fermentation broth: the fermentation medium is nutrient broth, and Brevibacterium cold-tolerant ZQ1-1 strain is picked and inoculated into the fermentation medium, and after 48 hours of cultivation, it becomes the fermentation broth, which is ready for use.

[0034] Preparation of kaolin suspension: Weigh 5g of kaolin into 950mL of deionized water, then add 50mL of 0.01g / mL CaCl 2 Solution, mixed evenly to form a stable suspension, ready to use.

[0035] Experimental group (A1): 2 mL of the above fermentation liquid was added to 100 mL of the above kaolin suspension; control group (A0): an equal amount of deionized water was added to replace the fermentation liquid. Treat the mixed solution at 180rpm for 1min, then at 8...

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Abstract

A Brevibacterium frigoritolerans strain capable of denitrification and efficient flocculation and application thereof are disclosed. The strain is named as Brevibacterium frigoritolerans ZQ 1-1 with an accession number of GDMCC No:60520. The stain has efficient flocculation capability and high ammonia nitrogen removing capability, wherein the flocculation rate of the strain for a kaolin suspensionhaving a concentration of 5 g / L in 10 min reaches 90% or above; in a medium the initial ammonia nitrogen concentration of which is 150 mg / L, the NH<+><4> nitrogen removal rate of the strain is 87% orabove; in pig manure slurry the initial ammonia nitrogen concentration of which is as high as 600 mg / L, the ammonia nitrogen removing rate of the strain is 60% or above, and nitrite nitrogen is not accumulated during ammonia nitrogen removing, thus avoiding secondary pollution. The strain can be used for water denitrification.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of cold-resistant Brevibacterium with denitrification and high-efficiency flocculation capabilities and its application. Background technique [0002] At present, my country's aquaculture industry is developing rapidly in the direction of scale and intensification, which not only promotes the development of agricultural economy and improves the quality of life of the people, but also brings many environmental problems and ecological crises. According to the Bulletin of the First National Survey of Pollution Sources, the annual discharge of ammonia nitrogen from the livestock and poultry industry in my country alone is as high as 650,000 tons and 1,024,800 tons of total nitrogen. Nitrogen-containing wastewater enters the surface water body and groundwater layer through surface runoff and soil infiltration, causing eutrophication of water body, death of aquatic orga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/13C02F101/16C02F101/10C02F103/20
CPCY02W10/10
Inventor 朱红惠陈猛李安章谢小林陈美标
Owner GUANGDONG BOWOTE BIOTECH CO LTD
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