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Method for screening population genome latent important gene based on MK-test

A genome and gene technology, which is applied in the field of potentially important gene screening of population genome based on MK-test, can solve the problems of not being able to determine the function of a single gene, heavy workload, etc., and achieve the effect of reasonable design and convenient operation

Active Publication Date: 2019-04-26
中国科学院昆明植物研究所海盐工程技术中心
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Problems solved by technology

However, these methods have a large workload for the screening of potentially important genes in population genomes, and cannot determine the function of individual genes in plants.

Method used

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  • Method for screening population genome latent important gene based on MK-test
  • Method for screening population genome latent important gene based on MK-test
  • Method for screening population genome latent important gene based on MK-test

Examples

Experimental program
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Embodiment 1

[0063] A method for screening potentially important genes in population genomes based on MK-test, including:

[0064] 1) Build a gene interaction network: collect gene interaction data from BioGRID, IntAct, DIP, MINT and STRING databases, separate the interaction data of target plants, and establish corresponding databases;

[0065] 2) Use the processing module to obtain and / or input gene parameters as required, wherein the processing module includes at least two gene chips, and a computer connected to the gene chip; the gene parameters include: species, population genes, difference nucleotide numbers between genes, gene total number of nucleotides;

[0066] 3) Output the inter-species and / or intra-species variation rate after the gene parameters are calculated by the standard formula, and screen out the potentially important genes of the population genome;

[0067] 4) Functional analysis of potentially important genes;

[0068] The above calculations use MK-test to calculat...

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Abstract

The invention provides a method for screening a population genome latent important gene based on MK-test, wherein the method belongs to the field of gene screening technology. The method comprises thesteps of 1), constructing a gene interacting network; 2), acquiring and / or inputting a gene parameter according to the requirement by means of a processing module; 3), outputting an inter-species and / or intra-species variation rate after operation of the gene parameter through a standard formula, and screening the latent important gene of the population genome; and 4), performing functional analysis on the latent important gene, wherein the processing module comprises at least two gene chips, and a computer which is connected with the gene chips. The method can realize large-batch simultaneous analysis to the inter-species or intra-species variation. After operation, the change trend of a non-expression segment proportion and / or expression segment proportion along with a gene change can be effectively analyzed, and an effective approach is supplied for latent important gene screening of the population genome.

Description

technical field [0001] The invention relates to the technical field of gene screening, in particular to a method for screening potentially important genes of population genomes based on MK-test. Background technique [0002] Molecular population genetics is the pillar subject of contemporary evolutionary biology research, and also the basic theoretical subject of genetic breeding, genetic association mapping and linkage analysis. Molecular population genetics is developed on the basis of classical population genetics. It uses the variation pattern of macromolecules, mainly DNA sequences, to study the genetic structure of the population and the relationship between the factors that cause population genetic changes and the genetic structure of the population. Scientists can accurately infer the evolution of populations quantitatively, which not only overcomes the limitation that classical population genetics can only study short-term changes in population genetic structure, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/50
Inventor 章成君金桂花石勇胡彦婷李玲
Owner 中国科学院昆明植物研究所海盐工程技术中心
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