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Maltose functionalized nanometer composite material based on ion complementation type peptide self-assembly and preparation method and application of composite material

A nanocomposite material and nanomaterial technology, applied in the field of maltose functionalized nanocomposite materials, can solve problems such as strong surface dependence, cumbersome processing steps, and harsh conditions, and achieve simple preparation methods, environmentally friendly processes, and mild conditions Effect

Active Publication Date: 2019-04-23
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, almost all functional groups reported are stepwise chemically bonded to the surface of nanomaterials, which requires cumbersome processing steps, strong surface dependence, and harsh conditions (non-aqueous solvents, high temperatures, etc.)

Method used

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  • Maltose functionalized nanometer composite material based on ion complementation type peptide self-assembly and preparation method and application of composite material
  • Maltose functionalized nanometer composite material based on ion complementation type peptide self-assembly and preparation method and application of composite material
  • Maltose functionalized nanometer composite material based on ion complementation type peptide self-assembly and preparation method and application of composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Synthesis of maltose-modified ion-complementary peptides

[0040] (1) Add 0.174g Fmoc-Rink Amide MBHA Resin into the solid-phase synthesis tube, add 5mL of dichloromethane to soak the resin for 30 minutes, fully swell, remove the dichloromethane by suction filtration, add 5mL of piperidine and N , The volume ratio of N-dimethylformamide is a mixed solution of 1:4, react for 20 minutes, remove the mixed solution by suction filtration, and then add 5 mL of piperidine and N,N-dimethylformamide with a volume ratio of 1: 4, reacted for 20 minutes, filtered with suction, washed the resin twice with dichloromethane, and washed the resin three times with N,N-dimethylformamide, 5 mL each time, to complete the Fmoc-Rink Amide MBHA Resin de-Fmoc-two Once, add 5mL N,N-dimethylformamide, 0.1331g Fmoc-Lys(Boc)-OH, 0.03837g 1-hydroxybenzotriazole, 0.1077g benzotriazole-N,N,N′ , N′-tetramethylurea tetrafluoroboric acid, 99 μL N,N′-diisopropylethylamine, stirred at room temperature ...

Embodiment 2

[0053] In this example, using C@Fe 3 o 4 Nanoparticles (prepared according to the method disclosed in the literature "Yang, J.; Li, J.; Qiao, J.; Lian, H.; Chen, H.J. Chromatogr. A 2014, 1325, 8-15") were replaced SiO in Example 1 2 @Fe 3 o 4 Nanoparticles, other steps are the same as in Example 1 to obtain maltose functionalized C@Fe 3 o 4 composite material.

[0054] Fe in above-mentioned embodiment 1 and 2 3 o 4 , SiO 2 @Fe 3 o 4 、C@Fe 3 o 4 , maltose functionalized SiO 2 @Fe 3 o 4 Composite materials and maltose functionalized C@Fe 3 o 4 Composite materials were subjected to Fourier transform attenuated total reflection infrared spectroscopy and X-ray photoelectron spectroscopy. The results are as follows: Figure 2 ~ Figure 6 shown. It can be seen from the figure that maltose functionalized SiO 2 @Fe 3 o 4 Composite materials and maltose functionalized C@Fe 3 o 4 Composite material (580cm -1 At Fe-O, 1100cm -1 Si-O-Si at 1740 and 1620cm -1 C=O a...

Embodiment 3

[0056] Application of the maltose functionalized nanocomposite obtained in Examples 1 and 2 in glycopeptide enrichment

[0057] 1. Contrast experiments on the enrichment performance of different materials

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Abstract

The invention discloses a maltose functionalized nanometer composite material based on ion complementation type peptide self-assembly and a preparation method and application of the composite material. By modifying self-assembly, on the surfaces of SiO2@Fe3O4, C@Fe3O4 and other nanometer materials, of the ion complementation type peptide with alternating hydrophilic and hydrophobic residues on maltose, the stable maltose functionalized nanometer composite material is obtained. The composite material can selectively enrich glycopeptides in standard glycoprotein and biological sample enzymolysisliquid, the enrichment efficiency is high, the selectivity is high, the detection limit is low, the performance is stable, and the composite material can be reused. The composite material is simple in preparation method, mild in condition, green, free of toxin and capable of being used for one-step surface functionalization of various nanometer enrichment materials.

Description

technical field [0001] The invention belongs to the technical field of surface functionalization of nanomaterials, and in particular relates to a maltose functionalized nanocomposite material based on ion-complementary peptide self-assembly, a preparation method of the material and an application of using the material to selectively enrich glycopeptides. Background technique [0002] Protein glycosylation is one of the most common and complex post-translational modifications involved in many biological processes, contains a lot of information related to mammalian diseases and genetic diseases, and is a hallmark of cancer and other diseases. Therefore, a comprehensive analysis of glycoproteins / glycopeptides is required for biological function and disease research. At present, mass spectrometry has become the most effective tool to provide sugar structure, glycosylation site and corresponding sugar chain heterogeneity in glycoproteomics research. However, the low natural abun...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K7/08C07K9/00
CPCC07K7/06C07K7/08C07K9/00
Inventor 党福全张莉李南石海兰
Owner SHAANXI NORMAL UNIV
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