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Preparation method of graphene quantum dots for Hg<2+> detection and tumor diagnosis

A graphene quantum dot and tumor diagnosis technology, applied in the chemical industry, can solve the problems of inconvenient detection of trace Hg2+, unsatisfactory requirements, large number of samples, etc., and achieve the effects of small background interference, good fat solubility, and simple preparation method

Inactive Publication Date: 2019-04-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These analytical methods have some disadvantages, such as high cost, large number of samples, complicated pretreatment, etc., which make trace Hg 2+ The inspection brings inconvenience
In recent years, graphene quantum dots have been widely used in the specific recognition of tumor cells and trace Hg 2+ However, the currently reported functionalized graphene quantum dots have weak functionality and cannot meet the needs. Therefore, it is of practical significance to synthesize functionalized graphene quantum dots with special structures that can improve selectivity while maintaining high sensitivity.

Method used

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  • Preparation method of graphene quantum dots for Hg&lt;2+&gt; detection and tumor diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 2g of citric acid and 0.005g of folic acid into a beaker, add 10mL of twice distilled water and stir until dissolved, heat, stir, and evaporate the water to dryness, and react at 200°C for 3 hours, and prepare the pH of the graphene quantum dots with sodium hydroxide solution For 6, the cultured normal cells and tumor cells were incubated in a solution containing 25ug / mL functionalized graphene quantum dots for 24 hours at a constant temperature of 37°C, and observed under a laser confocal microscope to achieve specific recognition of tumor cells.

Embodiment 2

[0025] Weigh 2g of citric acid and 0.01g of dihydrofolic acid into a beaker, add 10mL of twice distilled water and stir until dissolved, heat, stir, and evaporate the water to dryness, and react at 120°C for 2h, and use sodium hydroxide solution to dissolve the graphene quantum dots Prepare a pH of 6, and incubate the cultured normal cells and tumor cells in a functionalized graphene quantum dot solution containing 25ug / mL at a constant temperature of 37°C for 24 hours, and observe under a laser confocal microscope to achieve specific tumor recognition cell.

Embodiment 3

[0027] Weigh 2g of maleic acid and 0.03g of folic acid in a beaker, add 10mL of twice distilled water and stir until dissolved, heat, stir, and evaporate the water to dryness, and react at 150°C for 4h, and use sodium hydroxide solution to dissolve the graphene quantum The dot preparation pH is 6, and the cultured normal cells and tumor cells are incubated in the functionalized graphene quantum dot solution containing 25ug / mL at a constant temperature of 37°C for 24h, and observed under a laser confocal microscope to achieve specific recognition tumor cells.

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Abstract

The invention belongs to the field of chemical industry, and relates to a preparation method of graphene quantum dots for Hg<2+> detection and tumor diagnosis. The preparation method comprises: mixingan organic acid and a certain amount of azide-based functionalizing reagent, dissolving in water, stirring, and evaporating to obtain a mixture; carrying out a heating reaction on the prepared mixture at a certain temperature for a certain time to obtain functionalized graphene quantum dots; and dissolving the prepared functionalized graphene quantum dots in water, and carrying out tumor cell imaging or Hg<2+> concentration fluorescence determination. According to the present invention, the functionalized graphene quantum dots are prepared by using the organic acid as the carbon source and using the azide-based compound as the functionalizing reagent through the one-step reaction so as to achieve the specific recognition of tumor cells, and the specific bonding of the Hg<2+> and the functionalized graphene quantum dots can produce significant fluorescence quenching so as to achieve the highly-sensitive and highly-selective determination of the Hg<2+> concentration in the water sample.

Description

technical field [0001] The invention belongs to the field of chemical industry and relates to a Hg 2+ Preparation method of graphene quantum dots for detection and tumor diagnosis. Background technique [0002] Graphene quantum dots are a type of zero-dimensional graphite nanocrystals with a size smaller than 100 nanometers, which have strong quantum confinement and edge effects. Graphene quantum dots have the characteristics of low toxicity, high luminous intensity, good photostability, good biocompatibility, and adjustable bandgap, and are widely used in many aspects such as electrochemical sensors, optical sensors, and biological imaging (F.X.Wang, Z.Y.Gu, W.Lei, W.J.Wang, X.F.Xia, Q.L.Hao, Graphene quantum dots as a fluorescent sensing platform for highly efficient detection of copper(II)ions, Sens.Actuators B 190(2014)516-522). At present, the preparation methods of graphene quantum dots mainly include top-down synthesis method and bottom-up synthesis method. The for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/184C09K11/65G01N21/64
CPCC09K11/65G01N21/6402G01N21/6428G01N21/6458G01N2021/6432
Inventor 李在均汪漩
Owner JIANGNAN UNIV
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