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Thyroid cancer related markers and their application

A technology of thyroid cancer and drugs, applied in the field of biomedicine, can solve the problems of no effect and poor curative effect of thyroid cancer

Active Publication Date: 2022-04-12
思泰得精准(北京)医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both radiotherapy and chemotherapy have poor or no effect on thyroid cancer

Method used

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  • Thyroid cancer related markers and their application
  • Thyroid cancer related markers and their application
  • Thyroid cancer related markers and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening for Gene Markers Related to Thyroid Cancer

[0038] 1. Sample collection

[0039]From October 2014 to January 2018, 25 tissue samples were obtained from the Department of Endocrinology, Beijing Luhe Hospital, all of which were confirmed by pathological examination, including 8 paracancerous tissue samples and 17 thyroid cancer samples, numbered Store in a -80°C low temperature refrigerator. All the above specimens were obtained with the consent of the organizational ethics committee.

[0040] 2. Total RNA extraction from tissue samples

[0041] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0042] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0043] ① Add Trizol and ...

Embodiment 2

[0053] Example 2 Real-Time PCR Verification of GGTLC3 and LINC02560 Expression in Thyroid Cancer Tissue and Paracancerous Tissue

[0054] 1. Materials

[0055] 21 cases of thyroid cancer tissue samples and 8 cases of paracancerous tissue samples were selected and grouped and numbered. All samples were confirmed by pathological examination.

[0056] 2. Method

[0057] 2.1 Extract the total RNA from the tissue sample, the same as the extraction method in Example 1.

[0058] 2.2 Synthesis of cDNA by reverse transcription

[0059] use III Reverse Transcriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0060] Using a reverse transcription kit, 1 μg of total RNA was reverse-transcribed with reverse transcription buffer to synthesize cDNA. A 25 μL reaction system was used, and 1 μg of total RNA was used as template RNA for eac...

Embodiment 3

[0077] Example 3 RNAi interferes with the expression of GGTLC3 and LINC02560 and its effect on thyroid cancer cells

[0078] 1. Materials

[0079] 1. Source of cells

[0080] Thyroid cancer cell line SW579 was purchased from ATCC, USA.

[0081] 2. siRNA design and synthesis

[0082] According to the online design software siDirect version 2.0 (http: / / design.rnai.jp / ), according to GGTLC3 (ENSG00000274252) and LINC02560 (ENSG00000268307), the corresponding siRNA was designed, and the specific sequence is shown in Table 3. After the design is sent to the synthesis company for synthesis.

[0083] Table 3 siRNA sequence list

[0084]

[0085] 2. Experimental method

[0086] 1. Experimental grouping

[0087] Blank control group; liposome transfection group; non-specific siRNA-NC transfection group; specific GGTLC35-siRNA or LINC02560-siRNA transfection group.

[0088] 2. Transfection

[0089] Follow Lipofectamine TM The steps provided by 2000Transfection Reagent were ca...

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Abstract

The invention discloses the application of GGTLC3 and LINC02560 genes in the preparation of drugs for treating thyroid cancer, the drugs target GGTLC3 and LINC02560 genes; and provides an siRNA molecule for treating thyroid cancer, the siRNA molecules include inhibiting GGTLC3 and LINC02560 siRNA for gene expression. The present invention has discovered a molecular marker GGTLC3 and LINC02560 related to thyroid cancer, and the application of the inhibitor of the marker in the preparation of drugs for the treatment of thyroid cancer; Cancer gene therapy provides a new direction.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to markers related to thyroid cancer and applications thereof. Background technique [0002] The thyroid gland is an important endocrine organ in the human body, secreting hormones related to metabolism such as heart rate, blood pressure, body temperature and weight. Thyroid cancer (thytoid carcinoma, TC) is a common malignant endocrine system tumor of the head and neck developed from thyroid follicular cells, accounting for about 3% of the total number of malignant tumors in the whole body, accounting for the first place in the incidence of malignant tumors of the head and neck , mainly occurs in young adults, is common in people aged 45-54, and is more common in women over the age of 45, with a male to female ratio of about 1:3. In recent years, the incidence of thyroid cancer has shown an upward trend. In 2012, more than 40,000 women and more than 10,000 men in the United States were ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113A61K45/00A61K31/713A61P35/00
CPCA61K45/00A61P35/00A61K31/713C12N15/113C12Q1/6886C12N2310/141C12Q2600/118C12Q2600/178
Inventor 赵冬熊慧姚广印李丽纪智礼杨龙艳孙荣欣
Owner 思泰得精准(北京)医学检验实验室有限公司
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