Composite nanomicelle for multimodal treatment of nasopharyngeal carcinoma and its preparation method and application

A nano-micelle and nasopharyngeal cancer technology, applied in photodynamic therapy, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of difficult enrichment of target tissues and limited applications, and achieve good biodegradability properties, ease of chemical modification, and good biocompatibility

Inactive Publication Date: 2021-05-14
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrophilicity of Rose Bengal makes it difficult to accumulate in the target tissue, which greatly limits its application in photodynamic and sonodynamic therapy.

Method used

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  • Composite nanomicelle for multimodal treatment of nasopharyngeal carcinoma and its preparation method and application
  • Composite nanomicelle for multimodal treatment of nasopharyngeal carcinoma and its preparation method and application
  • Composite nanomicelle for multimodal treatment of nasopharyngeal carcinoma and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of composite nanomicelles for multimodal treatment of nasopharyngeal carcinoma:

[0026] 1) Mix the amphiphilic peptide ultrapure aqueous solution with the Bengal Rose Bengal ultrapure aqueous solution and place it in an ultrasonic cleaner; under the conditions of a temperature of 20 degrees Celsius and a power of 28 kHz, fully mix for 20-40 minutes to obtain a composite amphiphilic peptide nanomicelle product; wherein, the concentration of the amphiphilic peptide ultrapure aqueous solution is 10 mg / ml; the concentration of the described rose bengal ultrapure aqueous solution is a solution of 2 mg / ml. The volume ratio of the amphiphilic peptide ultrapure aqueous solution to the Rose Bengal ultrapure aqueous solution is 2:1.

[0027] 2) Transfer the above composite amphiphilic peptide nanomicelles obtained in step 1) to a dialysis bag with a molecular weight cut-off of 1000 Daltons, and dialyze for 48 to 72 hours to obtain the composite amphiphilic...

Embodiment 2

[0028] Example 2: Preparation of composite nanomicelles for multimodal treatment of nasopharyngeal carcinoma:

[0029] 1) Mix the amphiphilic peptide ultrapure aqueous solution with Bengal Rose Bengal ultrapure aqueous solution and place it in an ultrasonic cleaner; at a temperature of 5 degrees Celsius and a power of 15 kHz, mix thoroughly for 20-40 minutes to obtain a composite amphiphilic peptide nanomicelle Product; wherein, the concentration of the amphiphilic peptide ultrapure aqueous solution is 5 mg / ml; the concentration of the described rose bengal ultrapure aqueous solution is a solution of 0.5 mg / ml. The volume ratio of the amphiphilic peptide ultrapure aqueous solution to the rose bengal ultrapure aqueous solution is 1:1.

[0030] 2) Transfer the above composite amphiphilic peptide nanomicelles obtained in step 1) to a dialysis bag with a molecular weight cut-off of 800 Daltons, and dialyze for 48 to 72 hours to obtain a composite amphiphilic peptide nanomicelle ta...

Embodiment 3

[0031] Example 3: Preparation of composite nanomicelles for multimodal treatment of nasopharyngeal carcinoma:

[0032] 1) Mix the amphiphilic peptide ultrapure aqueous solution with the Bengal Rose Bengal ultrapure aqueous solution and place it in an ultrasonic cleaner; at a temperature of 28 degrees Celsius and a power of 30 kHz, mix thoroughly for 20-40 minutes to obtain a composite amphiphilic peptide nanomicelle Product; wherein, the concentration of the amphiphilic peptide ultrapure aqueous solution is 15 mg / ml; the concentration of the described rose bengal ultrapure aqueous solution is a solution of 2.5 mg / ml. The volume ratio of the amphiphilic peptide ultrapure aqueous solution to the Rose Bengal ultrapure aqueous solution is 2:1.

[0033] 2) Transfer the above composite amphiphilic peptide nanomicelles obtained in step 1) to a dialysis bag with a molecular weight cut-off of 1500 Daltons, and dialyze for 48 to 72 hours to obtain a composite amphiphilic peptide nanomic...

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Abstract

The invention belongs to the field of compound micelle-encapsulated medicine preparation, and in particular relates to a composite nano-micelle for multi-modal treatment of nasopharyngeal carcinoma, a preparation method and application thereof; the composite nano-micelle for multi-modal treatment of nasopharyngeal carcinoma, which is characterized in that: Including rose bengal and amphiphilic peptide nanomicelles for encapsulating the rose bengal. In this application, the amphiphilic peptide of C18-GRRRRRRRRGDS containing the arginine-glycine-aspartic acid tripeptide sequence is self-assembled into nanomicelles, and Bengal red is contained inside; the results show that: when injected intravenously, the combined treatment group It has a significant inhibitory effect on CNE‑2Z tumors. During intratumoral injection, the photodynamic therapy group with laser alone (RBNs+Laser), the sonodynamic therapy group with ultrasound alone (RBNs+US), and the combined therapy group (RBNs+Laser+US) all played a role in tumor growth. obvious inhibitory effect.

Description

technical field [0001] The invention belongs to the field of preparation of compound micelle-loaded drugs, and in particular relates to a compound nano-micelle for multi-mode treatment of nasopharyngeal carcinoma and its preparation method and application. Background technique [0002] Nasopharyngeal carcinoma is one of the most common malignant tumors in southern my country. It mainly occurs in Guangdong Province and Guangxi Zhuang Autonomous Region, and it brings great harm to the health of the people in high-incidence areas. Radiation therapy is the first choice for the treatment of nasopharyngeal carcinoma without distant metastasis, and it has a good control efficiency for early nasopharyngeal carcinoma. For advanced nasopharyngeal carcinoma, radiotherapy combined with chemotherapy is often used clinically. However, the acute toxic side effects associated with radiation therapy and chemotherapy drugs have relatively large toxic side effects on the human body, and some ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K47/42A61K41/00A61P35/00
CPCA61K9/1075A61K41/0033A61K41/0057A61K47/42A61P35/00A61K2300/00
Inventor 刘哲
Owner TIANJIN UNIV
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