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Method for artificially raising larvae of hepialus armoricanus oberthur

A technology for the host of Cordyceps sinensis and bat moths, which is applied in the biological field, can solve the problems of low survival rate and high incidence of bat moth larvae of the host of Cordyceps sinensis, and achieves the effects of improving the feeding efficiency and avoiding the occurrence of diseases.

Active Publication Date: 2019-04-16
CHANGXING PHARMA
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Problems solved by technology

[0006] In order to solve the fundamental problem of the high incidence rate and low survival rate of the larvae of the host moth of Cordyceps sinensis artificially raised at low altitude, the present invention provides a method for artificially feeding the larvae of the host moth of Cordyceps sinensis with the inorganic mineral vermiculite instead of meadow soil as the feeding substrate

Method used

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  • Method for artificially raising larvae of hepialus armoricanus oberthur
  • Method for artificially raising larvae of hepialus armoricanus oberthur
  • Method for artificially raising larvae of hepialus armoricanus oberthur

Examples

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Effect test

Embodiment 1

[0034] Embodiment 1 uses vermiculite as the method for raising the bat moth larvae artificially

[0035] Collect the eggs laid by adult bat moths after mating, sterilize with 0.1% sodium dichloroisocyanurate for 1 minute, and then place them in a petri dish with wet filter paper (no clear water on the filter paper), and carry out moisturizing maintenance in an environment of 15-16°C . The egg stage is about 35 days at 15°C. One week before hatching, eggs were put into an insect rearing box filled with a rearing substrate to raise, and the selected insect rearing box was 300mm×400mm×160mm. The thickness of the vermiculite as the insect culture substrate in the box is 2 cm, and the water content of the vermiculite is 100% (that is, 1000 grams of vermiculite is mixed with 1000 mL of 0.2% sodium dichloroisocyanurate solution). Put feed carrots on the vermiculite, and put 5-6 carrots in the insect rearing box. If you put 5 carrots, put 4 strips of filter paper with a width of abo...

Embodiment 2

[0038] The only difference from Example 1 is that carrots are replaced by Polygonum viburnum as the natural feed for bat moth larvae. The results showed that using carrots as feedstuffs grew faster than using natural feedstuff Polygonum villiformis, and the individual weight.

experiment example 1

[0039] Experimental example 1 Identification of Cordyceps sinensis host bat moth

[0040] In Example 1, the larvae of the host bat moth with vermiculite as the substrate were bred until the middle and late December, and the larvae began to pupate. After more than 30 days, they emerged from the pupal stage, and the pupae and adults were taken for species identification. The instrument used was: PCR instrument S1000TM The malCycler; using Sanger sequencing 3730×I sequencer; using MEGA 7.0, IQ-tree, NCBI, BOLD SYSTEMS analysis.

[0041] According to the comparison results of the NCBI and BOLD SYSTEMS databases, the identification of the mitochondrial COI gene sequence and the mitochondrial Cytb gene sequence showed that the similarity between the adult sample and the Hepialus xiaojinensis Tu, Ma et Z hang 2009 was 99%, and it could be identified as the Hepialus xiaojinensis Tu. Moth Hepialus xiaojinensis. According to the comparison results of the NCBI and BOLDSYSTEMS databases,...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a method for artificially raising larvae of hepialus armoricanus oberthur. The method uses inorganic mineral vermiculite instead of meadow soil as a feeding substrate to artificially raise the larvae of the hepialus armoricanus oberthur, and the method has the advantages that pathogens cannot survive in the inorganicvermiculite, pathogen infection sources causing the devastating diseases of the host larva can be completely cut off, and the pathogens cannot infect the host larvae inhabiting the feeding substrate,thereby greatly increasing the survival rate of the larvae. The method can effectively prevent and cure diseases, greatly improve the feeding efficiency of the larvae of the hepialus armoricanus oberthur, and lay a solid foundation for providing larvae sources for large-scale artificial cultivation production of cordyceps sinensis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for artificially feeding the larvae of the host moth of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis (Berk.) G.H.Sung et al.) is a famous Chinese medicinal material and rare tonic produced in the Yunnan-Guizhou Plateau and the Qinghai-Tibet Plateau of China, and is a unique resource in China. As early as the 8th century AD, Tibetan medicine in my country included Cordyceps sinensis in the famous Tibetan medicine "Yuewang Yaozhen". In 1757, Wu Yiluo, a famous doctor in the Qing Dynasty, had made detailed records on the efficacy of Cordyceps sinensis in "Materia Medica Congxin". "Chinese Pharmacopoeia" records the functions and indications of Cordyceps sinensis: "Nourishing the lungs and kidneys, stopping bleeding and resolving phlegm. It is used for chronic cough and dyspnea, hemoptysis due to labor cough, impotenc...

Claims

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Application Information

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IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K20/28
CPCA01K67/033A23K10/30A23K20/28A23K50/90
Inventor 李智林陆佳欢肖延铭程根喜华超谈伟平
Owner CHANGXING PHARMA
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