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Oral vaccine for preventing H7N9 virus infection and preparation method thereof

A virus infection and vaccine technology, applied in the field of oral vaccines for preventing H7N9 virus infection and their preparation, can solve problems such as poor safety, poor effectiveness and timeliness

Inactive Publication Date: 2019-04-12
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The safety of the H7N9 avian influenza vaccines reported for human use is relatively poor, and the effectiveness and timeliness of those with better safety are often poor

Method used

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  • Oral vaccine for preventing H7N9 virus infection and preparation method thereof
  • Oral vaccine for preventing H7N9 virus infection and preparation method thereof
  • Oral vaccine for preventing H7N9 virus infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Preparation of H7N9 oral vaccine of the present invention and its effect verification

[0038] 1. Method

[0039] This application is based on the a-lectin surface display system of Saccharomyces cerevisiae, according to AH-H7N9 cross-reactive HA (gene bank number: CY187618) (Special note: HA contains part of the HA1 fragment (C277-C346) and the entire HA2 Fragment, gene total length: 867bp), nucleoprotein (NP) (GenBank number: CY187621, gene length: 1497bp) or ion channel protein (M2) (GenBank number: CY187619.1, gene length: 291bp) at the end ( C-terminus or N-terminus) in different free states will produce different immune efficiencies. AH-H7N9 protein is displayed on the surface of Saccharomyces cerevisiae EBY100 through pYD1. At this time, the C-terminus of AH-H7N9 protein is in a free state. Yeast C-terminal surface display system-based H7N9 influenza vaccine (such as figure 1 shown). Similarly, the AH-H7N9 protein is displayed on the surface of Sa...

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Abstract

The invention discloses an oral vaccine for preventing H7N9 virus infection and a preparation method thereof. Specifically, the present invention discloses a recombinant plasmid, and also discloses recombinant yeast comprising the recombinant plasmid, and an application of the recombinant yeast in preparing the vaccine for preventing H7N9 virus infection. The present invention successfully establishes the recombinant yeast EBY100 / pYD1-HA comprising partial gene segments of H7N9 virus, confirms that HA can be efficiently expressed in yeast, and further proves that the recombinant yeast has goodimmunoprotective effect against the H7N9 virus, can be prepared into the oral vaccine, and has excellent application prospects.

Description

technical field [0001] The invention relates to an oral vaccine for preventing H7N9 virus infection and a preparation method thereof. Background technique [0002] In March 2013, the Chinese Center for Disease Control confirmed that a new H7N9 subtype of avian influenza virus had appeared in mainland China. As of February 25, 2016, there were 1,295 cases of human infection with H7N9 avian influenza virus in mainland China, of which 249 died, with a fatality rate of 20%. Like other A-type avian influenza viruses, the H7N9 subtype has a genome composed of 8 negative-strand single-stranded RNA fragments, encoding 10 viral proteins, of which the main surface antigen components are hemagglutinin (HA) and neuramin Acidase (neuramidinase, NA). Although the H7N9 virus does not yet have the ability of human-to-human transmission, because it can specifically bind to the α2-6 sialic acid receptor of the human upper respiratory tract, it occurs when the H7N9 virus continues to mutate,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/81C12N1/19A61K39/145A61P31/16C12R1/865
CPCA61K39/12A61K2039/542A61P31/16C12N7/00C12N15/81C12N2760/16122C12N2760/16134
Inventor 雷涵芩黔鸿林文杰张炜
Owner SOUTHWEST JIAOTONG UNIV
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