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Preparation method of fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles

A technology of imprinted polymers and epitopes, applied in the direction of fluorescence/phosphorescence, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of low therapeutic effect, large side effects, functional monomers and carriers Single and other problems, to achieve the effect of simple operation, high selectivity, and improve the effect of specific recognition

Inactive Publication Date: 2019-04-12
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the limitations of the synthesis method, the single functional monomer and carrier used, the function of imprinted polymer materials has certain limitations; and some materials use physical adsorption to load drugs, which have large side effects and low therapeutic effects. Enrichment control of therapeutic reagents is important

Method used

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  • Preparation method of fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles
  • Preparation method of fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles
  • Preparation method of fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles

Examples

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Embodiment 1

[0021] A method for preparing a fluorescent double-template epitope imprinted polymer based on silicon nanoparticles, using fluorescent nanoparticles coated with Si NPs as a carrier, linear nonapeptide in the extracellular region of HER2 overexpression and drug doxorubicin (DOX) is used as a double template, and the method of double template imprinting, epitope imprinting and surface imprinting is used to obtain imprinted polymers, including the following steps:

[0022] 1) Add 0.3180g of sodium citrate as a reducing agent to 8mL of glycerol, blow in argon and stir, add 3mL of silicon source N-(2-aminoethyl)-3-aminopropyltrimethoxy after 20min Silane (DAMO); after 10 minutes, the reaction precursor solution was transferred to an atmospheric pressure microwave reactor at a temperature of 180°C for 15 minutes, and the obtained orange-yellow solution was transferred to a dialysis bag with a molecular weight cut-off of 1KDa, and dialyzed for 24 hours to obtain Si NPs aqueous solut...

Embodiment 2

[0032] A method for preparing a fluorescent double-template epitope imprinted polymer based on silicon nanoparticles, the preparation steps are basically the same as in Example 1, the difference is that the quality of zinc acrylate in step 3) is 20 mg, and the fluorescent type Dual template imprinted polymers.

Embodiment 3

[0034] A method for preparing a fluorescent double-template epitope imprinted polymer based on silicon nanoparticles, the preparation steps are basically the same as in Example 1, the difference is that the quality of zinc acrylate in step 3) is 40 mg, and the fluorescent type Dual template imprinted polymers.

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Abstract

The invention relates to a preparation method of a fluorescent double-template antigenic-determinant imprinted polymer based on silicon nanoparticles. The fluorescent nanoparticles are taken as a carrier, and linear nonapeptide and adriamycin of an extracellular region where HER2 is over expressed are used as double templates to synthesize the imprinted polymer. The method comprises the followingsteps: preparing fluorescent silicon nanoparticles (Si NPs), carrying out silanization on the Si NPs; and preparing the fluorescent double-template antigenic-determinant imprinted polymer based on thesilicon nanoparticles. According to the method, the imprinted polymer is synthesized by combining antigenic-determinant imprinting, double-template imprinting and a surface imprinting method, the operation is simple and convenient, the process saves time, and the material has a targeting imaging function and a targeted therapy function. Zinc acrylate and acrylamide are adopted as functional monomers, and the templates are jointly fixed through metal chelating and action of hydrogen bonds, so that the specific recognition effect of the material on a target object can be effectively improved, and controlled release of medicines can be realized by utilizing an acidic pH value. The synthesized fluorescent imprinted polymer efficiently combines high sensitivity of fluorescence and high selectivity of a molecular imprinting technology.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, in particular to a method for preparing a silicon nanoparticle-based fluorescent double-template epitope imprinted polymer. Background technique [0002] Molecularly imprinted polymers are artificially synthesized antibodies that respond specifically to target molecules, and match template molecules in shape, size, and functional groups in the spatial structure. See: Wulff, G. Angew. Chem. Int. Ed. 1995, 34, 1812-1832; Liu, Z. Anal. Chem. 2014, 86, 12382-12389. Molecular imprinting technology has the advantages of low cost, good polymer stability and strong selective recognition ability, and has been widely used in the specific recognition and separation of small molecules. In order to solve the problems in the process of imprinting biomacromolecules, such as proteins due to their complex spatial structure, large size, and variability, methods such as surface imprinting and epitope imprin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F220/56C08F222/14C08J9/26A61K47/04A61K47/32A61K31/704G01N21/64
CPCA61K31/704A61K47/02A61K47/32C08F220/06C08J9/26G01N21/6402G01N21/6486C08F220/56C08F222/102
Inventor 李文友王海燕张玉奎何锡文
Owner NANKAI UNIV
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