Fluorescent silk making method based on spanish mackerel organ and folium mori composite carbon dots

Abstract

The invention discloses a fluorescent silk making method based on spanish mackerel organ and folium mori composite carbon dots. The fluorescent silk making method comprises the following steps: (1) preparing biocompatibile composite fluorescent carbon dots, namely preparing and extracting fluorescent carbon dots from spanish mackerel organs and folium mori by using a hydrothermal synthesis method;(2) breeding silkworms, namely spraying the prepared water-soluble fluorescent carbon dots on fresh folium mori, feeding 5 instar larva of silkworms with the folium mori, and feeding the composite fluorescent carbon dots into silk glands of the silkworms by virtue of absorption and conversion of the silkworms selves; and (3) collecting fluorescent silk, namely continuously feeding the silkworms with the folium mori with the fluorescent carbon dots till the silkworms spin and cocoon, and collecting silkworm cocoons after cocooning, thereby obtaining fluorescent silk. By adopting the method disclosed by the invention, no dip dyeing step is implemented in the whole process, no adhesive is used, tedious steps of water washing, drying and the like are avoided, under the condition that the natural quality of silk is not damaged, green preparation of the fluorescent silk is achieved, and silk with fluorescent properties can be made.

Description

technical field [0001] The invention relates to a method for preparing fluorescent silk based on composite carbon dots of Spanish mackerel viscera and mulberry leaves. Background technique [0002] Silk has been widely used due to its good mechanical properties, excellent biocompatibility and good biodegradability. In recent years, the research on natural silk modified materials has gradually attracted people's attention. More and more researchers are maintaining In addition to the original characteristics of natural silk, more new functions are introduced into silk, such as the preparation of fluorescent silk, which combines the good mechanical properties of silk and good biodegradability in vivo with the fluorescence detection method with high sensitivity, good selectivity, and strong operability Combining such advantages as the preparation of fluorescent silk can be applied in the field of biomedicine, such as cancer treatment with controlled release characteristics, prep...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for preparing fluorescent silk, such as: coating method, adsorption method, sol-gel method, layer-by-layer assembly method, genetic engineering transformation method, etc. The coating method usually requires exogenous fluorescent substances such as fluorescent dyes Or fluorescent nanoparticles are physically adsorbed and cross-linked to the surface of silk through binders, but organic dyes will pollute the environment; the adsorption method uses intermolecular hydrogen bonds, van der Waals forces and ionic bonds to make fluorescent substances adsorb to the surface of silk fibers, and wash with water The process is easy to cause the fluorescent substance to fall off; the sol-gel method and the layer-by-layer assembly method also face the stability problem of the fluorescent substance, and the preparation process is long, cumbersome and laborious; the silkworm genetic engineering modification method: many research groups have introduced the fluorescent protein gene into In the silkworm, try to use silkworm as a bioreactor to produce recombinant protein, so as to obtain fluorescent silk

For example, using genetic engineering technology to inject a virus loaded with green fluorescent protein into female silkworm moths, modify the heavy chain of silk fibroin to obtain fluorescent silk (Bioinspiration & biomimetics, 2018, 13, 041003), although the modified silkworm gene It is a more effective method for preparing fluorescent silk, but due to the complicated process and high cost of this method, it cannot be industrialized

Images