Markers and kits for predicting the sensitivity of HER2-positive metastatic breast cancer patients to trastuzumab
A technology of metastatic breast cancer and a kit, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve problems such as poor prognosis and failure to reflect the actual condition of the whole body of tumor patients, and achieve prediction Sensitivity, extraction and detection protocols are simple and easy, and the effect of less time spent
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Embodiment 1
[0029] Among 103 patients with HER2-positive advanced metastatic breast cancer treated with trastuzumab, 61 were sensitive to treatment and 52 were resistant. In these two groups of patients, through microRNA chip detection, the results are as follows figure 1 As shown, it was found that in tolerant patients, the expression of 3 microRNAs was up-regulated and the expression of 9 microRNAs was down-regulated. Among them, the expression of miR-940 increased, and the expression of miR-451a, miR-16-5p, and miR-17-3p decreased.
Embodiment 2
[0031] 1. Experimental method
[0032] 1. Selection of patients with HER2-positive metastatic breast cancer and collection of blood samples
[0033] Patient selection criteria: 1) pathological diagnosis of ductal invasive breast cancer; 2) immunohistochemical HER2 staining 3 (+), or in situ hybridization Her2 / CEP17 ratio ≥ 2.0); 3) expected survival time ≥ 3, physical status ECOG Score ≤ 2; 4) Can accept blood test.
[0034] Blood sample collection: Centrifuge the whole blood immediately after being collected in the anticoagulant tube, centrifugation conditions: 820g, 4°C, 10min; after centrifugation, pipette the serum into a new EP tube for RNA extraction.
[0035] 2. Addition of external reference and extraction of serum RNA;
[0036] cel-miR-39 was selected as the external reference microRNA, and 2.5ul cel-miR-39 (100nM) was added to every 500ul serum so that the concentration in each sample was 0.05nM. For RNA extraction, a kit from NorgenBiotek (Plasma / Serum Circulatin...
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