Colored-leaf forest seed mutagenesis method based on high-voltage electrostatic field
A high-voltage electrostatic field and colorful leaf technology, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, and plant phenotype improvement, can solve problems such as difficulty, high cost, and long growth cycle of colorful leaf trees, and achieve cost-effective Low cost, reduced breeding cost and shortened breeding cycle
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Embodiment 1
[0034] Embodiment 1 takes blue fir breeding as an example
[0035] S1: Seed selection: Select high-quality blue fir seeds with good maturity and full grains.
[0036] S2: High-voltage electrostatic field treatment: the intensity is 600KV / m, and the time is 24h.
[0037]1) Upper threshold for experimental determination: Under the same electric field strength of 600KV / m, set the following 10 time periods for electric field treatment: 0.1, 0.5, 1, 2, 4, 8, 12, 24, 48, 96 hours . 100 seeds are selected for each period of time and placed in a germination vessel, and then placed in a high-voltage electrostatic field processor for processing. After the treatment, soak the seeds, sow the seeds, observe and record according to the following steps S3-S5.
[0038] Experimental results: in the 7 stages before 24 hours, the blue fir seeds did not respond significantly to the electric field, and the germination rate did not change much; in the 24 hours stage, the germination rate was sup...
Embodiment 2
[0045] Embodiment 2 is an example with the breeding of Sophora japonica
[0046] S1. Seed selection: select high-quality Sophora japonica seeds with good maturity and full grains.
[0047] S2. High-voltage electrostatic field treatment: the intensity is 600KV / m, and the time is 2h;
[0048] 1) Upper threshold for experimental determination: Under the same electric field strength of 600KV / m, set the following 10 time periods for electric field treatment: 0.1, 0.5, 1, 2, 4, 8, 12, 24, 48, 96 hours . 100 seeds are selected for each period of time and placed in a germination vessel, and then placed in a high-voltage electrostatic field processor for processing. After the treatment, soak the seeds, sow the seeds, observe and record according to the following steps S3-S5.
[0049] Experimental results: in the 3 stages before 2 hours, the seeds of Sophora japonica did not respond significantly to the electric field, and the germination rate did not change much; in the stage of 2 h...
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