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A method for increasing the content of rare saponins in ginseng water extract

A water extract and rare saponin technology, applied in the field of fermentation, can solve the problems of low conversion efficiency, high cost, single content of rare saponins, etc., and achieve full utilization of raw materials, high conversion efficiency, and advantages in the number and content of rare saponins Effect

Active Publication Date: 2021-07-30
SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing reports are to ferment ginseng total saponins or monomeric common saponins. The raw materials fermented by these methods must be extracted and purified from ginseng or purchased separately, and the cost is relatively high; while direct fermentation with ginseng water extract, the current method Although the content of rare saponins is increased to a certain extent, the conversion efficiency is not high, and the content of rare saponins obtained is single

Method used

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  • A method for increasing the content of rare saponins in ginseng water extract
  • A method for increasing the content of rare saponins in ginseng water extract
  • A method for increasing the content of rare saponins in ginseng water extract

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Experimental program
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Effect test

Embodiment 1

[0035] (1) Strain activation: Sterilize the prepared MRS medium by high pressure steam, cool it to about 60°C and invert it on a plate, inoculate the Lactobacillus plantarum subsp. 24 hours. Acetobacter pasteurianus solid medium composition: 1% yeast extract, 1% glucose, 2% agar, 2% ethanol, pH 6.0, the percentages are mass percentages, and the balance is water. Inoculate the Acetobacter pasteuriani preserved on the slant on the solid medium of Acetobacter acetobacter, and incubate it upside down at 30°C for 24 hours.

[0036] (2) Seed culture: Take 250mL Erlenmeyer flasks, fill each bottle with 50mL MRS medium, cool after sterilization, pick a single colony of Lactobacillus plantarum subsp. Let stand for 24 hours. Acetobacter liquid medium formula: yeast extract 1%, glucose 1%, KH 2 PO 4 0.25%, ethanol 2%, pH6.0, percentage is mass percentage, balance is water; 250ml shaking flask is filled with 50mL of culture medium, picks single colonies of Acetobacter pasteuriani in ...

Embodiment 2

[0046] (1) Strain activation: Sterilize the prepared MRS medium by high pressure steam, cool it to about 60°C and pour it into a plate, inoculate the Lactobacillus plantarum subsp. plantarum subsp. Inverted culture at 35°C for 24 hours. Acetobacter pasteurianus solid medium composition: 1% yeast extract, 1% glucose, 2% agar, 2% ethanol, pH 6.0, the percentages are mass percentages, and the balance is water. Inoculate the Acetobacter pasteuriani preserved on the slant on the solid medium of Acetobacter acetobacter, and incubate it upside down at 30°C for 24 hours.

[0047] (2) Seed cultivation: take 250mL Erlenmeyer flasks, fill each bottle with 50mL MRS medium, cool down after sterilization, and inoculate single colonies of Lactobacillus plantarum and Lactobacillus fermentum in step (1) into the liquid medium Incubate at 35°C for 24 hours. Acetobacter liquid medium formula: yeast extract 1%, glucose 1%, KH 2 PO 4 0.25%, ethanol 2%, pH6.0, percentage is mass percent, and b...

Embodiment 3

[0057] (1) Strain activation: Sterilize the prepared MRS medium by high-pressure steam, cool it to a certain temperature and pour it on a plate, inoculate the Lactobacillus casei and Lactobacillus acidophilus preserved on the slope onto the MRS solid medium, and invert at 35°C Incubate for 24 hours. Acetobacter pasteurianus solid medium composition: 1% yeast extract, 1% glucose, 2% agar, 2% ethanol, pH 6.0, the percentages are mass percentages, and the balance is water. Inoculate the Acetobacterium preserved on the slant onto the Acetobacter solid medium, and culture it upside down at 30°C for 24 hours.

[0058] (2) Seed cultivation: Take 250mL Erlenmeyer flasks, fill each bottle with 50mL MRS medium, sterilize and cool down, pick the lactic acid colonies in step (1) and inoculate them in MRS liquid medium, and culture at 35°C for 24 hours. Acetobacter liquid medium formula: yeast extract 1%, glucose 1%, KH 2 PO 4 0.25%, ethanol 2%, pH6.0, percentage is mass percent, and b...

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Abstract

The invention belongs to the field of fermentation, and in particular relates to a method for increasing the content of rare saponins in water extracts of ginseng. The method comprises the following steps: using microwave ultrasonic co-extraction to prepare ginseng water extract, using the ginseng water extract as a fermentation substrate, and fermenting and transforming to produce four kinds of rare ginsenosides. By adjusting the compounding method of the starter and the conversion temperature, the content of characteristic rare saponins in the fermented liquid is greatly increased. The method has a simple process, and can increase the content of specific rare saponins through compound fermentation of strains under the condition that four kinds of rare saponins are simultaneously produced, and the conversion rate is high. It can be applied to ginseng deep-processing products with different needs.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a method for increasing the content of rare saponins in water extracts of ginseng. [0002] technical background [0003] Ginseng (Panax ginseng C.A.Mey) is the root of Araliaceae perennial plant ginseng. It is a traditional traditional Chinese medicine. Ginsenoside (ginsenoside GS) is the main material basis for the physiological activity of ginseng, and it is also the most effective medicinal ingredient in ginseng ingredients. Less rare ginsenosides (rare ginsenosides) have stronger biological activities and pharmacological effects, and have extremely high medicinal value and application prospects. For example, the rare ginsenoside Compound K (CK) has good effects on anti-tumor, anti-inflammation, anti-diabetes, anti-thrombosis, anti-aging and liver protection; Rg3 can induce tumor cell apoptosis, enhance the body's immunity, etc. effect. A variety of compound Chinese m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/225C12R1/02
CPCC12P33/20
Inventor 夏晚霞杨继国任杰王云刘玮刘丹
Owner SOUTH CHINA INST OF COLLABORATIVE INNOVATION
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