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Biological phenol-reducing microbial inoculum and application method thereof

A phenolic inoculant and biological technology, applied in the field of biological phenol-reducing inoculants, can solve problems such as difficulty in improving and restricting, and achieve the effects of reducing production and use costs, fast degradation, and convenient transportation and storage.

Inactive Publication Date: 2019-03-29
山东海景天环保科技股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the biological treatment technology is only limited to the treatment of low-concentration phenol-containing wastewater, and the high biological toxicity of high-concentration phenol-containing wastewater restricts the application of this technology, and in the existing technology, a single strain of phenol-containing wastewater is basically used to treat phenol-containing wastewater. , it is difficult to achieve a better improvement, so it is particularly important to isolate and breed strains or bacterial groups with high tolerance and degradation ability for bioaugmentation technology

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  • Biological phenol-reducing microbial inoculum and application method thereof

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Effect test

Embodiment 1

[0052] The acquisition of embodiment 1 bacterial strain

[0053] Sampling was taken from the soil near the phenol-containing sewage treatment system, and a total of 30 strains of single bacteria were isolated and screened, of which 14 strains had the ability to degrade volatile phenols;

[0054] From the 14 strains with the ability to reduce phenols, by increasing the concentration of phenol and studying its genetic stability and scope of application, two strains with high efficiency in degrading volatile phenols, easy to cultivate and stable inheritance were screened;

[0055] The inventors performed 16SrDNA sequencing on them, and their nucleotide sequences are shown as Seq ID No: 1 and Seq ID No: 2 respectively, which are the complete sequence of 16SrDNA of the strain. The measured 16SrDNA sequences were compared by BLAST, and at the molecular level, they were identified as Alcaligenes spp and Bacillus cereus, respectively,

[0056] The inventor named them YJY18-10 and YJY...

Embodiment 2

[0058] The acquisition of embodiment 2 bacterial powder

[0059] Utilize above-mentioned bacterial strain to obtain bacterial powder, the obtaining method of bacterial powder is as follows:

[0060] (1) Fermentation culture: Inoculate Alcaligenes and Bacillus cereus in the sterilized fermentation medium to obtain their respective bacterial liquids; at a temperature of 28-30°C, culture the fermenter until dissolved oxygen rises, pH falls;

[0061] (2) Preparation of bacterial powder: after centrifuging the fermented liquid, add auxiliary materials and mix evenly, and obtain solid bacterial powder after drying.

[0062] The more specific steps for the preparation of the two bacterial powders are as follows:

[0063] 1) Strain activation: On a sterile operating bench, take 1-5 μL of frozen-preserved Alcaligenes and Bacillus cereus in a test tube containing LB liquid medium, and culture at 28-32°C, 130-180rpm for 18-24h ;

[0064] 2) Seed preparation: transfer the activated st...

Embodiment 3

[0073] The utilization of embodiment 3 composite bacterial agents 1

[0074] The bacterial powder obtained in embodiment 2 needs to be activated in advance when used, and the activation method is:

[0075] Bacteria powder is inoculated in nutrient salts for activation according to the weight-to-volume ratio of 1-5g / L. The activation conditions are: 28-32°C, 130-180rpm shaker culture for 1-2h for activation.

[0076] Wherein the composition of nutrient salt solution is as follows: by weight:

[0077] 0.3-0.5 parts of dipotassium hydrogen phosphate; 0.01-0.02 parts of ferrous sulfate; 0.1-0.3 parts of calcium chloride; 0.3-0.5 parts of potassium dihydrogen phosphate; 0.1-0.5 parts of magnesium sulfate; 0.2 parts of sodium chloride; 0.5-1 parts of ammonium nitrate, 97-98.6 parts of demineralized water.

[0078] In the above activated salt solution, 300 mg / L of phenol was added in proportion as the only carbon source for activation treatment;

[0079] 1. Treated water quality: ...

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Abstract

The invention belongs to the technical field of biological engineering, and provides a high-efficiency biological phenol-reducing microbial inoculum and an application method thereof. The composite microbial inoculum consists of two strains. The strains are separated from a phenol-containing wastewater treatment system, and respectively are alcaligenes and bacillus cereus identified by 16SrDNA. The preservation numbers of the two strains are CGMCC NO.16158 and CGMCC NO.16157 respectively. When in use, according to the content of volatile phenol in inlet water in a sewage system, alcaligenes and bacillus cereus powder are mixed and activated according to a weight ratio of (1-3):1, then aerobic sludge is inoculated with the obtained bacterial according to a volume ratio of 1:(100-1000), andthe phenol-reducing capacity in 12 hours reaches 99%. The strains are easy to culture and propagate, and are prepared into solid bacterial powder which is convenient to transport and store. The amountand the proportion of the bacterial powder can be selected according to the water quality of inlet water when in use, and the strains are separated from a sewage treatment system and have the advantages of strong environmental adaptability, strong phenol resistance, high degradation concentration, high degradation speed and difficult secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and provides a biological phenol-reducing bacterial agent and an application method thereof. Background technique [0002] Phenol is widely used as a raw material in chemical production to make a variety of industrial products. In the process of producing these industrial products, phenolic wastewater with different pollution concentrations will be discharged. Phenol is a highly toxic substance, which can produce great biological toxicity to protoplasm. Different concentrations of phenol can denature and precipitate proteins to varying degrees. Phenols can cause direct damage to biological cells and are extremely corrosive to epithelial cells. Long-term contact or drinking water polluted by it will cause chronic poisoning; it will also affect the normal growth and reproduction of aquatic organisms, and when the concentration is high, it will even cause a large number of deaths of aquatic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/05C12R1/085C02F101/34
CPCC02F3/341C02F3/348C02F2101/345C12N1/20
Inventor 蔡倩倩吴文雷杨传伦车树刚张心青傅英旬马娜娜杨丹丹倪建龙魏征
Owner 山东海景天环保科技股份公司
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