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dc-cik co-cultured cells and its preparation method, sensitizing antigen and application

A DC-CIK and co-culture technology, applied in the field of immune cells, can solve the problems of poor anti-tumor killing effect and large limitations, and achieve the effects of facilitating clinical application, improving applicability, and improving efficiency

Active Publication Date: 2021-01-08
BEIHAO STEM CELL & REGENERATIVE MEDICINE RES INST CO LTD +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] Based on this, it is necessary to provide a DC-CIK co-cultured cell and its preparation method, sensitizing antigen and application to solve the problem of poor anti-tumor killing effect and large limitations of traditional DC-CIK co-cultured cells

Method used

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  • dc-cik co-cultured cells and its preparation method, sensitizing antigen and application
  • dc-cik co-cultured cells and its preparation method, sensitizing antigen and application

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preparation example Construction

[0039] The present invention also provides a preparation method of DC-CIK co-cultured cells, which includes the following steps:

[0040] Collecting mononuclear cells, respectively inducing and culturing DC cells and CIK cells, adding the above-mentioned sensitizing antigens for stimulating induction during the induction culture process of DC cells;

[0041] The DC cells induced by sensitizing antigen stimulation were co-cultured with CIK cells.

[0042] In a specific example, the mononuclear cells are collected from fresh peripheral blood or cord blood. Preferably, collect fresh peripheral blood or umbilical cord blood, add anticoagulant, avoid freezing or refrigeration during transportation, and conduct cell separation within 2 hours after collection; when separating, add peripheral blood or umbilical cord blood to a 50ml human lymphocyte separation tube , add 20ml to each separation tube, set the parameters at 20°C to 25°C, speed up and down at 9 and 0 respectively, and ce...

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Abstract

The present invention discloses a DC-CIK co-cultured cell, a preparation method thereof, a sensitizing antigen and an application thereof. The DC-CIK co-cultured cell is prepared by using separated mononuclear cells to be respectively induced and cultured into DC cells and CIK cells, wherein the DC cells are induced and stimulated by adding a self-constructed polypeptide sensitizing antigen whichis proven to be widely expressed in various tumor cells during a culture process, which can improve tumor killing effects. The DC-CIK co-cultured cell cultured by the method can have a very good killing effect on a variety of the tumor cells. Besides, the cultured DC-CIK cells have high activity and strong amplification ability, releases many cytokines of IFN-gamma, etc., and is strong in lethality. The co-cultured cell can be widely used in treatment of various cancers, including kidney cancers, prostate cancers, breast cancers, bladder cancers, melanoma, colon cancers, ovarian cancers, bonecancers, lung cancers, neuroblastoma, etc., and is wide in adaptive surfaces.

Description

technical field [0001] The invention relates to the field of immune cells, in particular to a DC-CIK co-cultured cell and its preparation method, sensitizing antigen and application. Background technique [0002] DC-CIK cell therapy is a cytokine-activated killer cell-dendritic cell mixed therapy, which contains both killer immune cells and dendritic cells. During tumor treatment, DC cells first recognize antigens and then activate the adaptive immune system; activated CIK cells efficiently kill tumor cells by exerting their own cytotoxicity and secreting cytokines. [0003] Dendritic cells (DC) are named for their dendritic or pseudopodia-like membranous protrusions on the cell surface when they mature, and are the most functional professional antigen-presenting cells. DCs originate from bone marrow CD34+HSC precursor cells, and are normally distributed in a small amount but widely in various organs of the body other than the brain, such as umbilical cord blood, human bone...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N5/0783C12N5/0784A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/4748C12N5/0638C12N2501/02C12N2501/22C12N2501/2301C12N2501/2302C12N2501/2304C12N2501/24C12N2501/25C12N2501/51C12N2501/515C12N2502/1121
Inventor 吴海涛沈政聂慧蓉施念黄春艳
Owner BEIHAO STEM CELL & REGENERATIVE MEDICINE RES INST CO LTD
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