Preparation method for self-assembly nanoparticle with targeting function and application thereof

A self-assembling nanoparticle and functional technology, applied in the field of biomedicine, can solve problems such as easy decomposition, and achieve the effect of simple and easy operation

Inactive Publication Date: 2019-03-26
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Epigallocatechin gallate (EGCG) is the most effective active ingredient in tea polyphenols. It is mainly distributed in green tea and belongs to catechins, but its properties are easy to decompose in acidic environment

Method used

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  • Preparation method for self-assembly nanoparticle with targeting function and application thereof
  • Preparation method for self-assembly nanoparticle with targeting function and application thereof
  • Preparation method for self-assembly nanoparticle with targeting function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of self-assembled nanoparticles UA@EGCG-Apt NPs with targeting function

[0031] Take 100 µL of 20, 10, 5, and 1 mM ursolic acid methanol solutions at room temperature, and slowly add them dropwise to 1 mL of water under ultrasonic conditions to completely volatilize the methanol, and then add 100 µL of 1 mM EGCG aqueous solution, ultrasonic for 45 minutes, to obtain UA@EGCG self-assembled nanocomposites, then add 10 µL 10 μM nucleic acid aptamer aqueous solution, shake on a shaker for 4-6 hours, and after standing at room temperature, you can get Function-oriented self-assembled nanoparticles UA@EGCG-Apt NPs.

Embodiment 2

[0032] Example 2 Particle size and potential analysis and detection of UA@EGCG self-assembled nanocomposites

[0033] The particle size distribution and Zeta potential of the nanocomposite prepared by the ratio of the amount of substances were measured using a Malvern particle sizer.

[0034] Table 1 Particle size distribution of UA@EGCG self-assembled nanocomposites with different molar ratios

[0035]

[0036] UA@EGCG self-assembled nanocomposites were prepared by "solvent exchange method". The ratio of substances is an important factor affecting the particle size of UA@EGCG nanocomposites, and the PDI value can reflect the distribution of nanoparticle sizes. UA@EGCG nanocomposites The optimum substance molar ratio was 10:1 (Table 1). When the molar ratio of substances is 1:1, the average particle size of UA@EGCG nanocomposites is 358.1 nm, the PDI is 0.376, and the potential is -27.3 mV; when the molar ratio of substances is 5:1, the UA@EGCG nanocomposites The average ...

Embodiment 3

[0037] Example 3 Agarose electrophoresis experiment

[0038] In order to verify whether Aptamer targeting tumor cell EpCAM can be adsorbed on the nano drug delivery system UA@EGCG self-assembled nanocomposite, an agarose electrophoresis experiment was carried out.

[0039] (1) Add 0.5 g of agarose to 50 mL of 0.5×TBE electrophoresis buffer and shake well. Heat in a microwave oven for 1 min until the agarose dissolves, take it out, shake it by hand, and continue heating in the microwave oven for 30 s until the agarose is completely dissolved to make a 1% agarose gel.

[0040] (2) Cool the dissolved agarose to about 60°C, pour it into the agarose gel mold with the comb inserted, cool and solidify at room temperature, and carefully pull out the comb vertically.

[0041] (3) Put the gel into the electrophoresis tank, add 0.5×TBE electrophoresis buffer solution into the electrophoresis tank until the liquid surface covers the gel by 1-2 mm.

[0042] (4) Sample treatment: 8 μL DNA...

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Abstract

The invention belongs to the technical field of biological medicines and specifically relates to a preparation method for a self-assembly nanoparticle with a targeting function and an application thereof. According to the invention, a solvent exchange method is adopted for preparing a self-assembly nano-composite and establishing a UA and EGCG self-assembly nanometer drug-carrying system with pH response and EpCAM receptor targeting ability, so that a nanometer drug delivery system according to the invention creatively utilizes a self-assembly technology to simultaneously prepare UA, EGCG andAptamer; the preparation process is simple and easy; a new research direction is supplied for oncotherapy.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of self-assembled nanoparticles with targeting function. Background technique [0002] Ursolic acid (Ursolic acid, UA), also known as ursolic acid, is widely found in hawthorn, ebony, Prunella vulgaris and other plants. , lowering blood sugar and other extensive biological effects. In addition, UA also has an obvious anti-tumor function, so its role has been valued by domestic and foreign pharmaceutical workers. The following formula is the chemical structural formula of ursolic acid. [0003] [0004] Epigallocatechin gallate (Epigallocatechin gallate, EGCG) is the most effective active ingredient in tea polyphenols. It is mainly distributed in green tea and belongs to catechin, but its property is easy to decompose in acidic environment. In recent years, studies have found that EGCG has antibacterial, antiviral, antiang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K31/353A61K31/56A61K47/54A61P35/00
CPCA61K31/353A61K31/56A61K47/549A61K47/6929A61P35/00A61K2300/00
Inventor 邵敬伟吴鹏宇张冰晨周敏
Owner FUZHOU UNIV
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