Super-lens microstructure generation method and micro two-photon microscope system based on super lens

Abstract

The invention provides a super-lens microstructure generation method. The method includes the following steps of generating phase maps, calculating phase distribution and generating microstructures. The invention further relates to a micro biphoton microscopy system based on the super-lens. A super-surface lens is introduced into the field of biphoton microscopy, medium numerical aperture focusingis achieved under full field of view, the structure of a microscope is greatly simplified, the weight of the overall equipment is largely reduced, and lighter load animal experiments can be achieved.The reliability of in vivo biphoton microscopy experimental data is improved, and the method has high scientific value for micro biphoton, especially in vivo microscopic imaging; the influence of a backpack type micro microscope system on observed objects (such as mice) is further reduced. According to the system, from simulation design to processing to experiments, a super-surface lens is introduced into the biphoton microscopy imaging system, and a new generation of imaging equipment is provided for brain imaging of living animals. The progress in brain and Neuroscience research is promoted.

Description

technical field [0001] The invention belongs to the technical field of in vivo visualized brain science research, and relates to a method for generating a superlens microstructure for deep imaging of living cells and a miniature two-photon microsystem based on the superlens. Background technique [0002] In vivo visual brain science research is the frontier field of life science research at home and abroad. In order to achieve neuron-level resolution, the coordinated application of various cutting-edge technologies such as micro-optics, micro-electro-mechanical systems, and biomarkers is required. As an effective means of deep imaging of living cells, two-photon fluorescence microscopy has been miniaturized to the size of a head-mounted mouse in the laboratory and applied to the imaging of neurons in the whole brain of mice. Miniature two-photon microscopes usually use gradient index lenses or multi-piece micro-objective lenses to scan and image samples. However, with the ...

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Problems solved by technology

[0007] In order to overcome the deficiencies in the prior art, the micro-two-photon microscope system based on the superlens proposed by the present invention solves the problem that the micro-two-photon microscope usually uses a gradient refractive index lens or a multi-piece micro-objective lens to scan and image the sample. The problem of further reducing the overall weight of the microscope under the condition of field of view and resolution

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