Using the p5126-zmms1m construct to create a maize dominant male sterile line and its application method for breeding and production

Abstract

The invention discloses a technological system for preparing a corn dominant genic male sterility line from a p5126-ZmMs1 and mCherry gene construct and a corn seed cross breeding and producing application method thereof. The construct contains three gene expression cassettes: an expression cassette for causing corn dominant genic male sterility, an expression cassette for marking corn peel colorand a weed killer resistance expression cassette. When the construct is guided into a corn callus tissue, the corn dominant genic male sterility line seeds can be prepared; under normal light, the transgenic sterility line seeds have no obvious difference from the non-transgenic fertile seeds, and goods quality is not affected; however, under green excitation light, the transgenic sterility line seeds show red fluorescence, and the non-transgenic fertile seeds show normal corn color and have no fluorescence. The technological system disclosed by the invention has qualitative difference from acorn recessive male sterility technology (namely a corn multi-control sterility technology system) which has been already applied for authorization by the team and can be efficiently applied to corn sterility cross breeding and hybrid seed production.

Description

technical field [0001] Generally, the present invention relates to the fields of molecular biology and plant genetic engineering and crop breeding. Specifically, the present invention relates to a method for creating a dominant nuclear male sterile line in maize and comprising a maize pollen-specific promoter p5126 , key genes of male flower development ZmMs1 and seed coat-specific fluorescent genes mCherry The construct, the recombinant vector comprising the construct, and the host cell transformed by the construct or the recombinant vector, the preparation method of the transgenic corn plant cell and the transgenic corn plant, and the corn male sterile line seeds produced therefrom And its hybrid breeding and seed production application method. Background technique [0002] Heterosis is the phenomenon in which a hybrid is superior to its two parents in one or more traits. For example, the first generation of hybrids obtained by crossing different strains, different va...

AI Technical Summary

Problems solved by technology

These methods have many disadvantages, such as high cost, unstable purity, and susceptibility to environmental influences, which restrict the application efficiency of heterosis in the actual production of corn.

[0005] Maize dominant male sterile materials do not have the problem of detasseling, but due to the lack of effective maintenance and reproduction techniques, it is difficult to apply them in actual production

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