Method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries
A polyphenol compound, separation and purification technology, applied in the preparation of organic compounds, the separation/purification of carboxylic acid esters, organic chemical methods, etc., to achieve the effects of being suitable for industrial production, simple and fast experimental process, and high separation efficiency
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[0042] 1. Prepare the extract
[0043] Take 0.5kg of the frozen fresh Baihua Sorbus fruit "Sorbus", mash it, add ethanol with a mass concentration of 80% according to the material-to-liquid ratio of 1kg:10L, and ultrasonically extract. The extraction temperature is 45℃ and the ultrasonic time is 1h. During this period, stir properly, filter statically, evaporate to dryness by rotating at 45°C, and then freeze-dry to obtain 87.9 g of sorbus fruit extract.
[0044] 2. Preliminary purification-resin column separation
[0045] Resin pretreatment: soak the polyamide in 95% ethanol solution for 24h, wash with distilled water until no alcohol smell; then soak in 2% NaOH solution for 24h, wash with distilled water until neutral; finally soak with 2% citric acid for 24h , Wash with distilled water to neutral.
[0046] Adsorption and elution: Take the pretreated 600mL polyamide and pack it into a chromatography column (4.5cm×50cm) to obtain a polyamide chromatography column. Weigh 20.0 g of t...
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