Method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries

A polyphenol compound, separation and purification technology, applied in the preparation of organic compounds, the separation/purification of carboxylic acid esters, organic chemical methods, etc., to achieve the effects of being suitable for industrial production, simple and fast experimental process, and high separation efficiency

Active Publication Date: 2019-03-22
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no application of high-speed countercurrent chromatography in the sepa

Method used

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  • Method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries
  • Method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries
  • Method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries

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Embodiment 1

[0042] 1. Prepare the extract

[0043] Take 0.5kg of the frozen fresh Baihua Sorbus fruit "Sorbus", mash it, add ethanol with a mass concentration of 80% according to the material-to-liquid ratio of 1kg:10L, and ultrasonically extract. The extraction temperature is 45℃ and the ultrasonic time is 1h. During this period, stir properly, filter statically, evaporate to dryness by rotating at 45°C, and then freeze-dry to obtain 87.9 g of sorbus fruit extract.

[0044] 2. Preliminary purification-resin column separation

[0045] Resin pretreatment: soak the polyamide in 95% ethanol solution for 24h, wash with distilled water until no alcohol smell; then soak in 2% NaOH solution for 24h, wash with distilled water until neutral; finally soak with 2% citric acid for 24h , Wash with distilled water to neutral.

[0046] Adsorption and elution: Take the pretreated 600mL polyamide and pack it into a chromatography column (4.5cm×50cm) to obtain a polyamide chromatography column. Weigh 20.0 g of t...

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Abstract

The invention discloses a method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries. The method comprises the steps as follows: mashing sorbus pohuashanensis berries, adding an ethanol solution for ultrasonic extraction, and performing concentration under reduced pressure to obtain crude extract of sorbus pohuashanensis berries; performing gradient elution by a polyamide chromatographic column with ethanol as an eluant, and performing concentration under reduced pressure to obtain eluates with different gradient fractions; separating and purifying the eluates with different gradient fractions with HSCCC (high-speed counter-current chromatography) to obtain neochlorogenic acid, chlorogenic acid, quercetin-3-O-(6''-alpha-L)-rhamnosyl-4'''-alpha-L-rhamnosyl)-beta-D-glucoside, 3,5-O-dicaffeoylquinic acid and rutin with purity higher than 95% respectively. The method adopts a simple process, is high in separation speed and low in comprehensive cost and has very good promotional and use values, and the product purity is high.

Description

Technical field [0001] The present invention relates to the technical field of separation and purification of compounds, in particular to a method for rapid separation and purification of polyphenol compounds in rowan fruit. Background technique [0002] Sorbus pohuashanensis Hedl. (Sorbus pohuashanensis Hedl.) is a plant of the genus Rosaceae Sorbus. Its fruit is called "Sorbus" and is widely distributed in Northeast and North China. The fruit is edible, and folks often use the fruit as medicine. Its fruit tastes bitter and sweet, and has a mild nature. It has anti-inflammatory, antitussive, anti-asthmatic, cardiac, anti-oxidant, anti-cancer and anti-radiation effects. It is a health product, food, Important industrial raw materials such as pharmaceuticals and cosmetics. Current research shows that rowan is rich in polyphenol compounds, which are its main active substances. Among them, the content of flavonoids is the most common berry, and its antioxidant activity (ABTS index...

Claims

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Application Information

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IPC IPC(8): C07C67/48C07C67/56C07C69/732C07H17/07C07H1/08
CPCC07B2200/07C07C67/48C07C67/56C07C69/732C07H1/08C07H17/07C07C2601/14
Inventor 刘玉峰孙珊珊朱丽君梁元昊胡瑞雪徐文丽马海燕李鲁盼
Owner LIAONING UNIVERSITY
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