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Moringa seed micro-molecule polypeptide and preparation process thereof

A technology of small molecular polypeptide and preparation process, which is applied in the field of small molecular polypeptide of Moringa seed and its preparation, and can solve the problems of poor absorption performance and low polypeptide content

Pending Publication Date: 2019-03-19
武汉天天好生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the current enzymatic hydrolysis method extracts Moringa oleifera seeds, the protein content in the product can be as high as 90%, but the polypeptide content in the protein is low, and the small molecular polypeptide content is even lower. Small molecular polypeptide refers to peptides with a molecular weight less than 1000D. Therefore, the absorbency of the product is poor

Method used

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  • Moringa seed micro-molecule polypeptide and preparation process thereof

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Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a small molecule polypeptide of Moringa oleifera seed, which is prepared according to the following preparation process:

[0052] Step 1. Moringa oleifera seed meal and water are in a mass ratio of 1:15, heated to 100°C, and homogenized for 1 hour to obtain a slurry.

[0053] Step 2, the slurry is cooled to 55° C., and under natural pH conditions, neutral protease solid powder accounting for 3% of the quality of Moringa oleifera seed meal and alkaline protease solid powder accounting for 3% of the quality of Moringa oleifera seed meal are added to the slurry, Enzymolysis was performed for 6 hours to obtain an enzymolysis solution.

[0054] Step 3. Inactivate the enzyme solution at 100°C for 15 minutes, and filter to obtain a filtrate.

[0055] Step 4. Concentrate the filtrate in vacuum at a vacuum degree of 0.065.

[0056] Step 5, spray drying, control the process parameters as follows: air inlet temperature 185°C, air outlet temperature 87°C,...

Embodiment 2

[0059] This embodiment provides a small molecule polypeptide of Moringa oleifera seed, which is prepared according to the following preparation process:

[0060] Step 1. Mix Moringa oleifera seed meal with water at a mass ratio of 1:10, heat to 90°C, and homogenize for 2 hours to obtain a slurry.

[0061] Step 2, cooling the slurry to 50° C., under natural pH conditions, adding neutral protease solid powder accounting for 4% of the mass of Moringa oleifera seed meal and alkaline protease solid powder accounting for 1% of the mass of Moringa oleifera seed meal in the slurry, Enzymolysis was performed for 6 hours to obtain an enzymolysis solution.

[0062] Step 3. Inactivate the enzyme solution at 100°C for 10 minutes, and filter to obtain a filtrate.

[0063] Step 4. Concentrate the filtrate in vacuum at a vacuum degree of 0.06.

[0064] Step 5, spray drying, control the process parameters as follows: air inlet temperature 180°C, air outlet temperature 70°C, wind-introduced r...

Embodiment 3

[0067] This embodiment provides a small molecule polypeptide of Moringa oleifera seed, which is prepared according to the following preparation process:

[0068] Step 1. Moringa oleifera seed meal is mixed with water at a mass ratio of 1:20, heated to 100°C, and homogenized for 0.5 h to obtain a slurry.

[0069] Step 2, cooling the slurry to 60° C., under natural pH conditions, adding neutral protease solid powder accounting for 1% of the mass of Moringa oleifera seed meal and alkaline protease solid powder accounting for 4% of the mass of Moringa oleifera seed meal in the slurry, Enzymolysis was performed for 6 hours to obtain an enzymolysis solution.

[0070] Step 3. Inactivate the enzyme solution at 100°C for 10 minutes, and filter to obtain a filtrate.

[0071] Step 4. Concentrate the filtrate in vacuum at a vacuum degree of 0.08.

[0072] Step 5, spray drying, control the process parameters as follows: air inlet temperature 190°C, air outlet temperature 95°C, wind-intro...

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Abstract

The invention discloses a moringa seed micro-molecule polypeptide and a preparation process thereof and relates to the field of deep processing of moringa seeds. The moringa seed micro-molecule polypeptide is mainly prepared by performing enzymolysis on moringa seed pulp and a complex enzyme under the natural pH condition, wherein the polypeptide content in the moringa seed micro-molecule polypeptide is more than 50%, and the content of peptide fragments having the molecular weight of less than 1000D in the polypeptide is more than or equal to 85%. The preparation process of the moringa seed micro-molecule polypeptide comprises the following steps: mixing moringa seeds and water, heating, and homogenizing to obtain slurry; cooling the slurry, and performing enzymolysis on the slurry, neutral protease accounting for 0.5-4% of the mass of the moringa seed pulp and alkaline protease accounting for 0.2-0.5% of the mass of the moringa seed pulp at a temperature of 50-60 DEG C under the natural pH condition for 2-8 hours so as to obtain enzymatic hydrolysate; performing enzyme deactivation on the enzymatic hydrolysate, filtering to obtain the filtrate, concentrating and drying the filtrate. The moringa seed micro-molecule polypeptide product is high in content of polypeptide, particularly high in content of micro-molecule polypeptide, and has excellent absorptivity.

Description

technical field [0001] The invention relates to the field of deep processing of moringa seeds, and in particular to a small-molecule polypeptide of moringa seeds and a preparation process thereof. Background technique [0002] Moringa is a plant of the genus Moringa in the Moringaceae family. It is native to the Himalayas in northern India and is widely planted in tropical and subtropical regions of Asia and Africa. The whole body of Moringa is full of treasures, and its leaves, flowers, seeds and tender buds, tender stems, roots, etc. are all edible, and it is a plant with the same origin of medicine and food. India and African countries commonly use Moringa to treat high-incidence diseases such as high blood pressure, diabetes, cardiovascular disease, rheumatism, and arthritis. Moringa seeds are rich in oil and protein, and are a high-quality source of protein. Moringa seed protein is rich in proton-donating or electron-donating amino acids, including: tyrosinase Tyr, me...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N9/54C12N9/24C12N9/42C12R1/125C12R1/07C12R1/10
CPCC12N9/2437C12N9/248C12N9/54C12P21/06C12Y302/01004
Inventor 陈大伟成静江方张涛
Owner 武汉天天好生物制品有限公司
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