Trace cell capturing system based on microfluidic and immune magnetic separation double policy

Abstract

The invention belongs to the technical field of cell separation and analysis, and in particular relates to a trace cell capturing system based on a microfluidic and immune magnetic separation double policy. The system comprises a spiral microfluidic chip and a chip tray comprising a magnet. The microfluid chip comprises a sample inlet (11), a sheath liquid inlet (12), a spiral runner (13), a tracecell collecting outlet (14) and a waste liquor port (15). The spiral runner is composed of a single spiral channel, the inlet of the single spiral channel is formed near the center of a round spiralchannel and enters the spiral microfluidic channel through a semi-circular starting channel, and the runner tail end comprises two outlets which separately collect trace cells and waste liquor in thesample. The chip tray is used for fixing a chip and comprises a chip tray base (21), a chip groove (22), a chip fixing elastic sheet (23) and a magnet (25). The round magnet is fixed in the groove ofthe chip tray and corresponds to the center position of the spiral runner. The trace cell capturing system provided by the invention combines the single spiral microfluidic chip and immune magnetic separation for the first time and can form a double policy cell separation system by means of cell sizes, density and surface markers, so that the recovery rate of trace target cells in a complex sampleis improved truly.

Description

technical field [0001] The invention belongs to the technical field of cell separation, and in particular relates to a method for capturing trace cells with dual strategies of microfluidic chip and immunomagnetic separation. Background technique [0002] Liquid biopsy technology selectively separates rare functional cells, especially circulating tumor cells (CTC) or ctDNA and other related cells and nucleic acid information, from the patient's peripheral blood, cerebrospinal fluid or bone marrow and other body fluids, and then analyzes them in combination with traditional detection techniques. Pathological diagnosis of patients provides biological information data for clinical diagnosis, condition monitoring and individualized treatment of tumors. Liquid biopsy complements traditional tissue biopsy techniques, avoiding invasive tissue sampling. [0003] However, since the content of target cells in body fluids is very small, usually 1-10 cells / mL, effective selective isolat...

AI Technical Summary

Problems solved by technology

However, the structure of the chip is complex, and the cost of processing and operation is relatively high.

[0005] Hou et al designed a helical microfluidic chip (H-W. Hou, M. E. Warkiani, Isolation and retrieval of circulating tumor cells using centrifugal forces, Sci Rep. 2013, 3, 1259.), using particles of different sizes and densities Due to the balance of inertial force and Dean force in the fluid, the distribution position in the flow channel is different, so that CTC can be effectively separated from whole blood, and the recovery rate of tumor cells can reach more than 80%. The chip structure is simple and the recovery efficiency is high. , less damage to cells, etc., but this method will miss tumor cells with small particle sizes

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