Biohybrid gradient hydrogel scaffold and its preparation method and application

Abstract

The invention discloses a biohybrid gradient hydrogel scaffold and its preparation method and application. The hydrogel is prepared from acryloyl glycinamide and N-acryloyl (trimethylol) aminomethane as raw materials, and the gel is melted , mixed with tricalcium phosphate, and 3D printed into a scaffold doped with tricalcium phosphate at the bottom and an upper hydrogel scaffold, using piezoelectric technology to print growth factors on the hydrogel scaffold layer. The doping of tricalcium phosphate increases the bone conduction ability of the scaffold and improves the mechanical strength of the scaffold. The hydrogel upper layer improves the lubricity of the cartilage replacement material, while the presence of factors promotes cartilage repair. The gradient scaffold of the present invention is integrally formed, has simple formula, safe ingredients, and mild preparation conditions, solves the problem of interfacial bonding between cartilage and subchondral bone, improves the ability of the gel-like cartilage repair material to bond with the host bone, and simultaneously loads phosphoric acid Tricalcium and growth factors improve the ability of cartilage and subchondral bone integrated repair.

Description

technical field [0001] The invention belongs to the field of hydrogel technology, and relates to a method for preparing a biohybrid gradient hydrogel scaffold for integrated repair of cartilage and subchondral bone, more specifically, to a method using acryloyl glycinamide and N- A method for preparing acryloyl (trihydroxymethyl) aminomethane copolymerized supramolecular hydrogel as a monomer, and a method for printing this hydrogel into a gradient scaffold by using an integrated printing technology. The forming method is simple, and the scaffold simultaneously loads β -TCP and TGF-β1, improve the ability of cartilage and subchondral bone integration repair. Background technique [0002] The prevalence of articular cartilage damage is relatively high. According to reports, about 60% of patients undergoing arthroscopic examination have articular cartilage damage in varying degrees, and the prevalence rate of people over 65 years old is as high as 60%-90%. However, since arti...

AI Technical Summary

Problems solved by technology

However, the growth factor itself has problems such as short half-life, easy inactivation, high price and risk of side effects, and the subsequent sterilization and storage of growth factor-loaded scaffolds are also difficult

Growth factors usually can only be loaded on the surface of the scaffold in a specific way after the scaffold is formed, and the process is complex and cumbersome

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