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Preparation method of novel Iota carrageenan oligosaccharide

A technology of carrageenan oligosaccharide and carrageenan, which is applied in the field of seaweed oligosaccharide extraction, and achieves the effects of facilitating industrialization and application, stable quality and high purity

Inactive Publication Date: 2019-03-08
QINGDAO BZ OLIGO BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the preparation of new Iota carrageenan oligosaccharides by enzymatic hydrolysis in the prior art, and the enzymatic degradation method can protect the active groups of the reaction substrate to the greatest extent from being destroyed during the degradation process, and the degradation products are uniform and the reaction conditions Mild, high product activity, so the development of enzymatic hydrolysis to prepare Iota carrageenan oligosaccharide technology, to achieve its high activity, large-scale preparation and popularization and application has become a technical problem to be solved in this field

Method used

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  • Preparation method of novel Iota carrageenan oligosaccharide
  • Preparation method of novel Iota carrageenan oligosaccharide

Examples

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Embodiment 1

[0027] A kind of preparation method of new Iota carrageenan oligosaccharide comprises the following steps:

[0028] (1) Culture and activation: inoculate the screened Cellulophaga sp.QY201 strains into the liquid culture medium with an inoculum size of 2%, culture them on a shaker at 20-30°C and 60-100rpm / min for 35 hours, and perform culture and activation to obtain Crude enzyme solution; the liquid medium is composed of the following components in mass percentage: 3%NaCl, 0.5%MgSO 4 ·7H 2 O, 0.02%CaCl 2 , 0.01%KCl, 0.002%FeSO 4 , 0.25%CaSein, 0.15%NaH 2 PO 4 , 0.2%NaNO 3 , 0.25% Iota carrageenan;

[0029] (2) Coarse separation of acetone precipitation: Slowly add 2.5 times the volume of acetone to the crude enzyme solution, let it stand for 4 minutes, refrigerate and centrifuge at 8000-10000r / min for 20-30min at 4°C, discard the supernatant, and dissolve the precipitate in 20mmol / L Glycine-sodium hydroxide buffer solution with pH 10, dialyze overnight, refrigerate and...

Embodiment 2

[0037] Example 2 Detection results and analysis of new Iota carrageenan oligosaccharides

[0038] (1) High performance liquid chromatography (HPLC) analysis

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Abstract

The invention discloses a preparation method of novel Iota carrageenan oligosaccharide and belongs to the technical field of seaweed oligosaccharide extraction. The method comprises the following steps: (1) performing Cellulophaga sp. QY201 strain fermentation to prepare a crude enzyme, (2) performing crude separation on an acetone precipitate, (3) performing ion-exchange column chromatography, (4) performing gel filtration chromatography to prepare carrageenanase, (5) dissolving Iota carrageenan with water, (6) performing Iota carrageenan enzymolysis, and (7) inactivating, centrifuging and freeze-drying enzymolysis liquid to prepare the novel Iota carrageenan oligosaccharide. The method can quickly and massively prepare the novel Iota carrageenan oligosaccharide; a prepared novel Iota carrageenan oligosaccharide product is uniform in distribution, mainly comprises novel Iota carrabiose, novel Iota carratetrose, novel Iota carrahexaose and novel Iota carraoctaose and is high in purityand strong in bioactivity; the method adopts enzymic degradation, so that a preparation process is simple, quick, mild in condition and stable in quality; the novel Iota carrageenan oligosaccharide can be prepared in large scale; and the high-purity carrageenan oligosaccharide product can be provided for research and development in the fields of medicine, agriculture, food and the like.

Description

technical field [0001] The invention relates to a preparation method of a new Iota carrageenan oligosaccharide, which belongs to the technical field of seaweed oligosaccharide extraction. Background technique [0002] As a natural marine sulfated polysaccharide with unique structure, carrageenan is called heparan-like polysaccharide, and its biological activity has become a hot spot in the field of polysaccharide research. In recent years, the biological activity of carrageenan in physiological and pathological research has been discovered one after another. Experimental studies have shown that carrageenan has biological activities such as anti-angiogenesis, anti-tumor, immune regulation, anti-oxidation, antibacterial, anti-virus, and hypoglycemic. However, due to the large molecular weight of carrageenan polysaccharide, poor solubility and absorption, complex structure and other reasons, its application is limited. The molecular weight of carrageenan oligosaccharides is s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12P19/00
CPCC12P19/00C12P19/04C12P19/14
Inventor 王丽丽张斌管昶岳帆高芹芹徐小龙
Owner QINGDAO BZ OLIGO BIOTECH
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