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Method for rapidly application of esteya vermicola solid culture product in prevention and treatment of pine wilt disease

A technology of amnicidal and solid culture of Izobacillus, which is applied in the field of microbial biological control, can solve the problems of reducing manpower and material resources, and that solid culture products cannot be directly applied to biological control, so as to reduce manpower, improve biological activity and prolong the storage period. Effect

Active Publication Date: 2019-02-22
成昌根
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the application, only drilling and nailing are required, and there is no need to use a high-pressure plug, which greatly reduces manpower and material resources; at the same time, the storage period of the product is greatly extended, and the biological activity of the preparation is significantly improved; it solves the problem that the solid culture product cannot be directly Difficulties applied to biological control

Method used

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  • Method for rapidly application of esteya vermicola solid culture product in prevention and treatment of pine wilt disease
  • Method for rapidly application of esteya vermicola solid culture product in prevention and treatment of pine wilt disease
  • Method for rapidly application of esteya vermicola solid culture product in prevention and treatment of pine wilt disease

Examples

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Effect test

Embodiment 1

[0034] A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0035] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0036] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 6 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus...

Embodiment 2

[0049] A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0050] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0051] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 5 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus...

Embodiment 3

[0064]A method for rapidly applying the solid culture product of Anidida iridii to prevent and treat pine wilt disease, comprising the pre-obtained Amynidida iridii bacterium colony, comprising the following steps:

[0065] (1) Pretreatment: Sterilize the operation room, ultra-clean workbench, solid stirring fermenter and all equipment used in subsequent steps, put all sterilized equipment into the ultra-clean workbench, and turn on the ultraviolet lamp , blast; operators wear sterilized overalls, caps, masks and gloves;

[0066] (2) Strain preparation: Weigh 27g of PDB powder, dissolve it in 1L of distilled water, and distribute it in a culture container, and sterilize it under high temperature and high pressure for 20 minutes; after cooling, insert the colonies of Animidilia eideri, and place it on a shaker for cultivation For 6 days, the culture temperature was 26°C, and the shaker speed was 120 rpm; after the culture was completed, the strains of Animidobacter isobacillus ...

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Abstract

The invention discloses a method for rapidly application of an esteya vermicola solid culture product in prevention and treatment of a pine wilt disease, and belongs to the technical field of biological control. According to the method, a certain proportion of nutrient substances, a protective agent, a growth stimulating factor, a spore stimulation factor and an adhesive are added into the solid culture product and are uniformly mixed, then a harmless plasticizer is added into the solid culture product, the solid culture product is pressed and processed into a long-rod-shaped product, finallya layer of wafer is wrapped around the surface of the long-rod-shaped product, the whole production process is isolated from the outside environment, manual operation is not needed, and therefore pollution caused by human factors or exposure to the external environment is reduced; during application, only two operation links of drilling and knocking-in are needed, a high-pressure plug does not need to be used, and therefore manpower and material resources are greatly reduced; meanwhile, the storage life of the product is greatly prolonged, and the biological activity of the product is remarkably improved; and the problem that a solid culture product cannot be directly applied to biological control can be solved. The method has the advantages of being simple in operation and low in cost when being used for the prevention and treatment work of woodland.

Description

technical field [0001] The invention belongs to the technical field of microbial biological control, and in particular relates to a method for preventing and treating pine wilt disease by using a solid fermentation product of the pine wood nematode parasitic fungus-Animidilia eziri. Background technique [0002] Pine wilt disease, also known as pine wood nematode disease, is a dangerous disease caused by pine wood nematode (Bursaphelenchus xylophilus) that causes rapid death and continuous destruction of pine trees. It is mainly transmitted by insects such as monochamus. The disease was first reported in Japan in 1905, and quickly spread to China, South Korea, Vietnam, Europe and other countries and regions, seriously threatening the safety of pine forests worldwide. The disease has the characteristics of multiple transmission routes, rapid onset, and difficult treatment. Up to now, there is still no effective prevention and treatment method. It is called pine cancer. my co...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00A01G7/06A01G13/00C12R1/01
CPCA01G7/06A01G13/00A01N63/00C12N1/20
Inventor 王海华殷灿王云波陶然高杰段贝贝王春燕王振成昌根
Owner 成昌根
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