Method for preparing pig corneal endothelium implant piece by adopting femtosecond laser technology for assistance

Abstract

The invention discloses a method for preparing a pig corneal endothelium implant piece by adopting a femtosecond laser technology for assistance. The method comprises the following steps that S1, a pig cornea is pretreated; S2, after the pig cornea enters an interactive interface of a femtosecond laser machine, surgical parameters of femtosecond lasers are set according to a pig cornea thickness value measured before surgery, the lasers are precisely gathered on a set corneal surface and flattened, and laser blasting and gasified cutting are conducted to complete manufacturing of a corneal posterior lamellum implant piece which is a corneal endothelium piece with a posterior elastic layer, wherein the thickness is 100 microns, and the diameter is 8.5 mm; S3, decellularization operation isconducted through physical methods such as swelling, repeated freezing and thawing, shaking and air drying; S4, the pig corneal endothelium implant piece is prepared. The method adopts an instantaneous laser blasting technology so that precise corneal cutting can be completed, the immunogenicity of a pig corneal matrix piece is reduced, and the ultra-thin pig corneal endothelium implant piece withthe posterior elastic layer can also be prepared at the same time, so that transplantation difficulties caused by nonuniform thickness are greatly reduced.

Description

technical field [0001] The invention relates to the field of preparation of corneal endothelial grafts, in particular to a method for preparing pig corneal endothelial grafts assisted by femtosecond laser technology. Background technique [0002] In recent years, tissue engineered (TE) cornea has made significant progress, and it has attracted much attention because it can greatly alleviate the problem of organ failure and shortage of transplanted donor organs. Since human corneal endothelial cells cannot be repaired after injury, how to It is particularly important to establish a corneal endothelial cell tissue in vitro that can perform biological functions similar to corneal endothelial cells to treat patients with endothelial blindness. Endothelial keratoplasty is the replacement of diseased endothelium with healthy donor tissue. It has been widely recognized as the only effective option for the treatment of corneal endothelial dystrophy. At present, whether it is penetra...

AI Technical Summary

Problems solved by technology

Endothelial keratoplasty is the replacement of diseased endothelium with healthy donor tissue. It has been widely recognized as the only effective option for the treatment of corneal endothelial dystrophy. At present, whether it is penetrating keratoplasty, or DSEK, DMEK and other surgical methods The improvement of corneal donors is required to provide corneal donors, which cannot fundamentally solve the problem of donor shortage. In recent years, endothelial cell transplantation has become a research hotspot, and significant progress has been made in the in vitro culture of autologous cells or allogeneic cells.