Targeted multifunctional nanoparticles and preparation method and application thereof

Abstract

The invention relates to targeted multifunctional nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines. The targeted multifunctional nanoparticles are prepared by an ultrasonic double-emulsification method, covalent binding is carried out by branching PEI and PLGA, PLGA nanoparticles which have negative charge originally are modified into kation nanoparticles with positive charge, plasmids can be adsorbed effectively, it ensures that the plasmid cannot drop due to physical effect in a circulation system, and after the plasmid enters cells, the possibility that the plasmid is degraded by endoenzyme is also reduced. The nanoparticles further contain IR780 which can target tumor cell mitochondria, meanwhile, by strong fluorescent light and photosensitizer characteristics of IR780, the nanoparticles become a great fluorescent and photoacoustic imaging medium, and thus, tumors can be convenient to observe. The nanoparticles can carry drug to facilitate combination with transgenic therapy, tumor cells are killed favorably, and furthermore, toxic and side effects of chemotherapy drugs can further be reduced. The preparation method of the targeted multifunctional nanoparticles is simple, is easy to operate, and is suitable for expanded production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a targeted multifunctional nanoparticle and its preparation method and application. Background technique [0002] Most small animal gene transfection uses direct injection of plasmids into the tail vein, but this method does not have the specificity and precision of the transfection site, and the injected plasmids cannot enter the cells well, and are easily degraded, resulting in low transfection efficiency And other defects, so it is not suitable for gene therapy of tumors. With nanoparticles as carriers, due to the EPR effect of tumors, nanoparticles themselves have a tendency to accumulate and phagocytize at tumor sites, and are often used as drug sustained-release carriers, so plasmids can be entrapped in the inner water phase of nanoparticles, but At present, there are still two disadvantages in encapsulating the plasmid in the inner water phase of the nanop...

AI Technical Summary

Problems solved by technology

[0002] Most small animal gene transfection uses direct injection of plasmids into the tail vein, but this method does not have the specificity and precision of the transfection site, and the injected plasmids cannot enter the cells well, and are easily degraded, resulting in low transfection efficiency and other defects, so it is not suitable for gene therapy of tumors

With nanoparticles as carriers, due to the EPR effect of tumors, nanoparticles themselves have a tendency to accumulate and phagocytize at tumor sites, and are often used as drug sustained-release carriers, so plasmids can be entrapped in the inner water phase of nanoparticles, but At present, there are still two disadvantages in encapsulating the plasmid in the inner water phase of the nanoparticle. One is that during the process of encapsulation, the synthesis of the nanoparticle requires the use of a sonic shock instrument, which is a kind of damage to the plasmid and makes it open. Second, the release efficiency of plasmids entrapped in the inner aqueous phase will be much lower than those adsorbed on the surface after entering the cells

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