Preparation method of culture medium for rapid propagation of jasminum sambac

Abstract

The invention provides a preparation method of a culture medium for rapid propagation of jasminum sambac, and belongs to the field of jasminum sambac propagation. The method comprises the following steps: 1. preparation of a primary culture medium: KT, CTK, saccharose, asparagus cochinchinensis extract and agar are sequentially added into a MS culture medium according to the formula until to be completely dissolved, pH value is adjusted, and then sterilization under high temperature is carried out; 2. preparation of a proliferation medium: the KT, ethylic acid, the saccharose and the agar aresequentially added into the MS culture medium according to the formula until to be completely dissolved, the pH value is adjusted, the sterilization under high temperature is carried out, and heteroauxin is added after cooling; 3. preparation of a rooting medium: the KT, the saccharose, coconut juice, tender willow juice and the agar are sequentially added into the MS culture medium according to the formula, the pH value is adjusted, and then the sterilization under high temperature is carried out. The invention has the beneficial effects that the preparation method is simple and easy to operate, low in cost and friendly to environment; different culture mediums are definitely provided for the rapid propagation of the jasminum sambac at different stages; different demands of the jasminum sambac in different energy substances and effective substances are met at the different propagation stages; seedlings are promoted to healthily grow; the seeding rate is increased; the seedlings are strong.

Description

technical field [0001] The invention belongs to the field of jasmine propagation, in particular to a preparation method for a medium for rapid propagation of jasmine. Background technique [0002] Jasmine [Jasminum sambac (L.) Aiton] is an evergreen shrub of the genus Jasminum in the family Oleaceae, with an upright or half-open crown, native to tropical and subtropical regions. There are more than 60 common jasmine varieties, such as creeping jasmine, woody jasmine, pearl jasmine, and foreign jasmine, which can be divided into three types: single-lobe jasmine, double-lobe jasmine, and multi-lobe jasmine according to their flower shape. China is the country with the largest jasmine production in the world, and its annual output accounts for more than 60% of the world's total production. Now it is widely planted in Guangxi, Fujian, Guangdong, Yunnan and other places. Among them, Guangxi is the largest production area of ​​jasmine in my country, accounting for 80% of the natio...

AI Technical Summary

Problems solved by technology

[0003] However, the jasmine cultivar is single. For a long time, only the local bilobal jasmine in Hengxian County, Guangxi has been cultivated, and the pests and diseases have gradually increased. The offspring are seriously separated, and cuttings are often used for production. However, long-term asexual reproduction leads to the degradation of jasmine species, the decline in stress resistance, and the decrease in flower production year by year.