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Feed ingredients comprising lysed microbial cells

A microbial cell and feed technology, applied in the field of feed ingredients containing lysed microbial cells, can solve the problem of increased availability of long-chain omega-3 fatty acids, and achieve the effect of reducing transportation and storage costs

Pending Publication Date: 2019-02-05
CORBION BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aquaculture is growing, but availability of long-chain omega-3 fatty acids from wild-caught fish has not increased with demand

Method used

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  • Feed ingredients comprising lysed microbial cells
  • Feed ingredients comprising lysed microbial cells
  • Feed ingredients comprising lysed microbial cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0090] Example 1. Food Composition

[0091] DHA-enriched Schizochytrium cells (biomass) prepared by standard heterotrophic fermentation were dried and loaded using a standard impeller type mixer at loading levels of 10%, 20% and 30% by weight biomass with low Erucic acid canola oil blend. The biomass in the oil blend is then processed through a high pressure homogenizer or bead mill to produce a dispersion of lysed algal cells in the oil. For lysis in a high pressure homogenizer, a pressure of 200 bar to 1200 bar is used and is sufficient to lyse the cells.

example 2

[0092] Example 2. Analysis of mixtures and dispersions prepared from mixtures

[0093] A 20% by weight biomass blend blended with the canola oil of Example 1 was prepared and analyzed by laser diffraction particle size analysis on a Malvern Mastersizer 3000. The mixture was also homogenized and bead milled at 400 bar, and the resulting dispersion was also analyzed. figure 1 It is shown that the bead milled dispersion has a lower average particle size than the homogenized dispersion, and both have a lower average particle size than the masterbatch.

[0094] exist figure 2 The smaller size can also be seen in the photomicrograph of (400x magnification). The Dv50 value of the mixture (distribution value of 50% or less) was 228 μm, while the homogenized dispersion had a Dv50 of 15.3 μm, and the bead-milled dispersion had a Dv50 of 3.8 μm, indicating that the dispersion has a better on the characteristics of the sprayer.

[0095] Measured with a Malvern Kinexus professional rh...

example 3

[0097] Example 3. Oxidation Stability Analysis

[0098] The dry biomass of Example 1 was combined with various antioxidants using a hand blender. Dried biomass with or without antioxidants was analyzed using Oxipress at about 80°C. Natural and synthetic antioxidants were used and included L-alpha-lecithin (soybean, Calbiochem #429415), starch (corn, Argo), ascorbic acid (Spectrum Chemical Co. )), ParadigmOx White Liquid (PWL-Gallate, Comming Health), RPT40 (Rosemary, Tocopherol, Ascorbyl Palmitate, Comming Health), NaturFortLGR105 (Green Tea and Rosemary Extract, Comming Health Bright Health), Rendox CQ (TBHQ, Comming Health), RendoxEQ (ethoxyquin, Comming Health), BHT (crystalline in ethanol, Comming Health), and Covi-ox T-30P ( mixed tocopherols). Figure 5 The surprising and unexpected oxidative stability of certain biomass-containing lecithins is shown.

[0099] The oxidative stability of the biomass was about 22 hours at 80°C when prepared with the antioxidant RPT40 (...

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Abstract

The present disclosure relate to feed ingredients and formulated feed, methods for their manufacture, and uses thereof in nutritional applications such as in aquaculture, terrestrial animal feed, andhuman nutrition. The feed ingredient compositions comprise lysed microbial cells with a small aspect ratio and triglyceride oil.

Description

[0001] Cross References to Related Applications [0002] This application claims priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application No. 62 / 357,829, filed July 1, 2016, and entitled "FEED INGREDIENTS CONTAININGLYSED MICROBIAL CELLS" rights, and claims to U.S. Provisional Patent Application No. 62 / 408,630, filed October 14, 2016 and titled "FEED INGREDIENTS CONTAINING OXIDATIVELY STABLE UNLYSED AND LYSED MICROBIALCELLS" ", and U.S. Provisional Patent Application No. 15 / 636,506 filed June 28, 2017 and entitled "FEED INGREDIENTSCOMPRISING LYSED MICROBIAL CELLS" pursuant to 35 U.S.C. §120 requirements priority interests, each of which is incorporated herein by reference in its entirety. Background technique [0003] Triglyceride oils produced by microorganisms and plants provide essential nutrients for consumption by higher organisms in the food chain. This triglyceride oil consists of certain fatty acids that have not been discovered or are produced in relat...

Claims

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Application Information

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IPC IPC(8): A23K10/00A23K10/16A23K10/18A23K10/20A23K10/22
CPCA23K10/00A23K10/18A23K10/20A23K10/22A23K20/00A23K20/158A23K50/80A23K10/16A61K31/202Y02A40/818A61K9/167A61K36/02
Inventor W·莱基特斯基J·皮朔基W·卢J·维J·加拉佐
Owner CORBION BIOTECH INC
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