A method for quantitative detection of L-pantoprazole and dex-pantoprazole in biological samples by ultra-high performance convergence chromatography-mass spectrometry
An ultra-efficient confluence and pantoprazole technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of incompatible mass spectrometry, narrow application range, high equipment and technical requirements, and achieve high sensitivity and small sample size. , without the effect of endogenous matrix interference
Active Publication Date: 2019-09-13
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES +1
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Problems solved by technology
Among them, both GC and HPCE have the advantages of high efficiency and high sensitivity, but the scope of application is narrow
The advantage of HPLC is that it is the most widely used, does not require high temperature, and reduces isomerization, but it is usually separated in normal phase mode. The mobile phase is usually n-hexane and isopropanol or other normal phase solvents, and is not compatible with mass spectrometry.
SFC has the advantages of high separation efficiency of GC and HPCE and wide application range of HPLC, but it needs to be operated under high pressure, which requires high equipment and technology
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Embodiment 1
[0059] Ultra-high performance convergence chromatography (UPCC) is combined with triple quadrupole mass spectrometry (MS / MS) to detect chiral drug L-pantoprazole and D-pantoprazole in plasma; detection method of the present invention is carried out specificity, Method validation of standard curve, lower limit of quantitation (LLOQ), residue, precision and accuracy, matrix effect, extraction recovery, and stability at autosampler temperature for 24 hours.
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Abstract
The invention provides a method for quantitatively detecting the levo-pantoprazole and the dextro-pantoprazole in a biological sample by means of ultra-high performance convergence chromatography-massspectrometry. According to the method, after the biological sample to be detected is subjected to pretreatment, the liquid to be detected is subjected to the ultra-high performance convergence chromatography-mass spectrometry detection; according to the peak area and the standard curve of a chiral drug enantiomer and an internal standard in a detection spectrum, the concentration of different enantiomers of the chiral drug in the biological sample to be detected is calculated through an internal standard method; the invention combines the ultra-high performance convergence chromatography (UPCC) with a triple quadrupole mass spectrometry (MS / MS) for quantitative detection of chiral drugs in biological samples for the first time; and the method provided by the invention is high in sensitivity, can meet the separation of chiral drugs in biological samples with low sample volume, low content, and complex matrix, and is short in detection time and low in cost.
Description
technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for quantitatively detecting L-pantoprazole and D-pantoprazole in biological samples by ultra-high performance convergence chromatography-mass spectrometry. Background technique [0002] Chiral drugs (Stereoisomeric Drugs) refer to drugs containing chiral centers or asymmetric centers in their molecular structures, including single enantiomers and mixtures of two or more enantiomers. The physical and chemical properties of these enantiomers are basically similar, only the optical activity is different. Studies have shown that about 60% of commonly used drugs have one or more chiral centers, and about 50% of the more than 2,000 commonly used drugs listed in the US Pharmacopoeia are chiral drugs. The chiral drug market is still expanding year by year, and chiral drugs have become an important direction for the research and development of new molecular entities. In...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
Inventor 杨威林俊粒张家伟盛亚丽汪巨峰
Owner GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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