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Oligosaccharide with insulin resistance activity intervention effects and preparation method thereof

A technology of insulin resistance and oligosaccharides, which is applied in fermentation and other directions, can solve the problems of ineffective prevention and treatment of diabetes, and achieve the effects of increasing economic added value, high efficiency, and easy industrial production

Inactive Publication Date: 2019-02-01
浦江县欧立生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The average molecular weight of the laminar oligosaccharides obtained through the pretreatment of raw materials, the preparation of crude enzyme solution, and the preparation of laminar oligosaccharides is 2846, and the average degree of polymerization is 7, but it has no effect on the prevention and treatment of diabetes

Method used

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  • Oligosaccharide with insulin resistance activity intervention effects and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0027] Oligosaccharides with the activity of interfering with insulin resistance and a preparation method thereof, including degreasing, deproteinizing, depigmentation, purification, enzymatic hydrolysis, and dialysis refining of Chinese yam, the specific steps are as follows:

[0028]Yam degreasing: wash, dry and pulverize the yam, add calcium lactate with 0.45% weight of yam powder, sodium benzoate with 1.5% weight of yam powder, add 70% ethanol aqueous solution, at 85°C, 800W, microwave-assisted extraction for 30min, Repeat the extraction 3 times, the dry powder weight to ethanol volume ratio (g / mL) is 1:15, the extract is centrifuged, the precipitate is collected, and the defatted yam is obtained;

[0029] Deproteinization: Add distilled water to the defatted yam, wherein the mass ratio of defatted yam to distilled water (g / mL) is 1:25, stir evenly, at 80°C, ultrasonically extract for 2 hours, filter, repeat the extraction three times, combine the filtrate, and decompress ...

Embodiment 2

[0035] Oligosaccharides with the activity of interfering with insulin resistance and a preparation method thereof, including degreasing, deproteinizing, depigmentation, purification, enzymatic hydrolysis, and dialysis refining of Chinese yam, the specific steps are as follows:

[0036] Yam degreasing: wash, dry and crush the yam, add calcium lactate with 0.4% weight of yam powder, sodium benzoate with 1% weight of yam powder, add 50% ethanol aqueous solution, at 80°C, 750W, microwave-assisted extraction for 15min, Repeat the extraction twice, the ratio of dry powder weight to ethanol volume (g / mL) is 1:10, the extract is centrifuged, and the precipitate is collected to obtain defatted yam;

[0037] Deproteinization: add distilled water to the defatted yam, wherein the mass ratio of defatted yam to distilled water (g / mL) is 1:20, stir evenly, at 70°C, ultrasonically extract for 1 hour, filter, repeat the extraction three times, combine the filtrate, and decompress Concentrate, ...

Embodiment 3

[0043] Oligosaccharides with the activity of interfering with insulin resistance and a preparation method thereof, including degreasing, deproteinizing, depigmentation, purification, enzymatic hydrolysis, and dialysis refining of Chinese yam, the specific steps are as follows:

[0044] Yam degreasing: wash, dry and crush the yam, add calcium lactate with 0.5% weight of yam powder, sodium benzoate with 2% weight of yam powder, add 80% ethanol aqueous solution, at 90°C, 900W, microwave-assisted extraction for 45min, Repeat the extraction 4 times, the dry powder weight to ethanol volume ratio (g / mL) is 1:10, the extract is centrifuged, the precipitate is collected, and the defatted yam is obtained;

[0045] Deproteinization: add distilled water to the defatted yam, wherein the mass ratio of defatted yam to distilled water (g / mL) is 1:30, stir evenly, at 90°C, ultrasonically extract for 3 hours, filter, repeat the extraction three times, combine the filtrate, and decompress Concen...

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Abstract

The invention discloses oligosaccharide with insulin resistance activity intervention effects and a preparation method thereof, and the preparation method comprises the following steps: Chinese yam degreasing, deproteinization, pigment removal, purification, enzymolysis and dialysis and refining, wherein the enzymolysis step is as follows: purified Chinese yam polysaccharide powder is dissolved bydistilled water, the pH value is adjusted, undecanol and petroleum ether are added, then polygalacturonase, beta-galactosidase, beta-mannase and beta-glucanase are sequentially added, ultrasonic-assisted enzymolysis, enzyme inactivation, cooling and centrifugation are carried out, and supernate is taken for later use. The preparation method disclosed by the invention has the beneficial effects that ultrasonic wave assistance is used, the polygalacturonase, beta-galactosidase, beta-mannase and beta-glucanase are sequentially utilized to carry out step-by-step enzymolysis on the Chinese yam polysaccharide, hydrophobic substances undecanol and petroleum ether are added, by cooperation with the cavitation effect of the ultrasonic wave, a complex bonding structure of the Chinese yam polysaccharide is fully digested, the enzymolysis efficiency is greatly improved, and the method has the advantages of being convenient to operate, high in efficiency and free of pollution.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to an oligosaccharide with the activity of interfering with insulin resistance and a preparation method thereof. Background technique [0002] Diabetes mellitus is a systemic metabolic disorder characterized by hyperglycemia caused by defects in insulin secretion and / or its biological function, and its incidence is increasing year by year. Yam is a traditional medicinal and edible plant, which has the functions of nourishing spleen, kidney, strengthening spleen and stomach, benefiting essence, stopping diarrhea, and reducing phlegm. Yam contains starch, protein, amylase, fat, polysaccharide, vitamins, amino acids and various trace elements . Yam polysaccharide is the main active ingredient of yam, mainly mannan, heteropolysaccharide, mucopolysaccharide, etc. It has immune regulation, hypoglycemic, anti-oxidation, anti-aging, anti-tumor effects. [0003] Compared with po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/04
CPCC12P19/14C12P19/04C12P19/12
Inventor 李冰
Owner 浦江县欧立生物技术有限公司
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