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Wheat endophyte and an application thereof

A geobacterial and fusarium technology, applied in application, bacteria, biocide and other directions, can solve the problem of less research on wheat endophyte

Active Publication Date: 2019-02-01
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on endophytic bacteria in crops mainly focuses on rice, potatoes, corn, etc., and the research on endophytic bacteria in wheat is less

Method used

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  • Wheat endophyte and an application thereof
  • Wheat endophyte and an application thereof
  • Wheat endophyte and an application thereof

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Experimental program
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Embodiment Construction

[0022] 1. Medium preparation:

[0023] (1) Enrichment medium:

[0024] 1) Solution 1: 0.1% sodium citrate 2-hydrate, 0.6% K 2 HPO 4 , 1.4% KH 2 PO 4 , 0.2% (NH4) 2 SO 4 ;

[0025] 2) Solution 2: 0.02% MgSO 4 ·7H 2 O;

[0026] 3) Solution 3: 1.1% α-chitin powder solution;

[0027] 4) Mix the above three solutions in a volume ratio of 89:1:10.

[0028] (2) Chitin solid medium:

[0029] Solution A 50ml, solution B 50ml, 100 times trace metal solution 10ml, 100 times vitamin mixed solution 10ml, yeast extract 1.0g, colloidal chitin 10g, agar 18g, add distilled water to 1000ml pH=7.0~7.2. Wherein, the specific components of each solution are shown in Table 1.

[0030] Table 1 Chitin solid medium composition

[0031]

[0032]

[0033] Preparation of colloidal chitin: Weigh 10g flake chitin into a Erlenmeyer flask, add 200ml concentrated hydrochloric acid, stir overnight on a magnetic stirrer, then hydrate and precipitate with water at 4°C, centrifuge at 4000r / min...

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Abstract

The invention belongs to the field of microbial application, and particularly relates to a wheat endophyte and an application thereof. The specific technical features are characterized in that the wheat endophyte is preserved on CGMCC on August 6th, 2018, and preserved at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, China, with a preservation number of CGMCC No. 13938. The endophyte can be applied to the control of plant diseases, especially for the control of wheat diseases and watermelon fusarium wilt. The invention provides a brand new species of the geobacter strain, which has obvious effects in controlling plant diseases, and may have other unknown effects. The geobacter strain has important promotion value for the healthy growth and yield increase of various economic crops, and has research significance and guiding value for studying the effect of fungi on plant diseases.

Description

technical field [0001] The invention belongs to the field of microbial application, and in particular relates to a wheat endophytic bacterium and its application. Background technique [0002] Plant disease is a major problem faced by plant cultivation, causing about 10% loss of global crop production every year. More than 80% of plant diseases are caused by fungi. At present, for such diseases, chemical control is mainly used, that is, chemically synthesized fungicides are used to eliminate and control fungal diseases. However, chemical fungicides rely on several limited modes of action. Long-term use not only requires continuous increase in dosage, but also easily makes pathogens resistant. In the past 40 years, the amount of chemical fungicides has tripled, which has seriously aggravated environmental pollution. In addition, chemical fungicides will also kill beneficial insects and beneficial microorganisms in the soil, and are likely to enter the food chain. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 代玉梅张玲利徐智斌樊小莉
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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