Phospholipid substance containing Omega-3 fatty acid and preparation method thereof

A technology of fatty acid and fatty acid acyl, applied in the field of biochemistry

Inactive Publication Date: 2019-01-29
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In order to solve the deficiencies of the above-mentioned prior art, the present invention provides a phospholipid and its preparation method to fill the blank of the current related phospholipid products and overcome the defects of the current phospholipid preparation process

Method used

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  • Phospholipid substance containing Omega-3 fatty acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Add 50g of soybean lecithin to 100g of refined fish oil ethyl ester (70% DHA content, 1.4% EPA content), stir and heat up to 100°C under vacuum conditions, maintain 10min with nitrogen to break the vacuum, add sodium ethoxide dropwise, and conduct a closed reaction under nitrogen conditions After 1 hr, the reaction was continued in vacuum for 3 hrs. After the reaction was completed, 200 mL of acetone was added, refrigerated and left to stand for crystallization overnight, vacuum filtered, and the filter cake was vacuum-dried to obtain 27.3 g of light yellow powder.

[0034] HPLC measures the content of phospholipids in the product, and the HPLC conditions are:

[0035] Chromatographic column: Si 60 chromatographic column, 5μ, 4.6×250mm;

[0036] Mobile phase: n-hexane / isopropanol / 1% acetic acid aqueous solution=8 / 8 / 1(V / V / V);

[0037] Flow rate: 1.0mL / min;

[0038] Column temperature: 30°C;

[0039] Detector: 210nm.

[0040] GC determination of the composition of fat...

Embodiment 2

[0043] Add 50g of soybean lecithin to 100g of refined fish oil ethyl ester (80% EPA content, 0.5% DHA content), stir and heat up to 130°C under vacuum conditions, maintain 10min with nitrogen gas to break the vacuum, add sodium ethoxide dropwise, and seal the reaction under nitrogen conditions After 0.5 hr, the reaction was continued in vacuum for 5 hr. After the reaction was completed, 200 mL of acetone was added, refrigerated and left to stand for crystallization overnight, vacuum filtered, and the filter cake was vacuum-dried to obtain 25.5 g of light yellow powder.

[0044] Utilize the method in " embodiment 1 " to measure phospholipid content and fatty acid composition in the product.

[0045] Measurement result shows: phospholipid content 88.2% in the obtained phospholipid thing, is made up of phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol; %.

Embodiment 3

[0047] Add 50g of soybean lecithin to 150g of refined fish oil methyl ester (95% DHA content, 0.7% EPA content), stir and heat up to 130°C under vacuum conditions, maintain 10min with nitrogen gas to break the vacuum, add sodium methoxide dropwise, and conduct a closed reaction under nitrogen conditions After 1 hr, the reaction was continued in vacuum for 4.5 hrs. After the reaction was completed, 250 mL of acetone was added, refrigerated and left to stand for crystallization overnight, vacuum filtered, and the filter cake was vacuum-dried to obtain 28.3 g of light yellow powder.

[0048] Utilize the method in " embodiment 1 " to measure phospholipid content and fatty acid composition in the product.

[0049] The measurement result shows: phospholipid content 81.6% in the obtained phospholipid thing, is made up of phosphatidic acid, phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol; DHA accounts for 89.6% of total fatty acid content, and EPA accounts for 0....

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Abstract

The invention provides a phospholipid substance containing Omega-3 fatty acid and a preparation method thereof. In the phospholipid substance, the content of phospholipid is more than 80%. Main fattyacid in the phospholipid is one of DHA or EPA, wherein the content of the main fatty acid is 50-90% and the content of the other one is less than 1%. The phospholipid substance is high in phospholipidcontent, high in Omega-3 fatty acid purity in the phospholipid, single in composition and clear in function, and can be applied in the field of specific medicine, dietary supplements and food additives. Meanwhile, the invention also provides a method for preparing the phospholipid substance. The method is simple in process and low in cost, is suitable for industrial production, and makes up for shortcomings of conventional preparation methods.

Description

technical field [0001] The invention relates to a phospholipid and a preparation method thereof, wherein the phospholipid content is more than 80%, and the main fatty acid in the phospholipid is one of DHA or EPA, belonging to the field of biochemistry. Background technique [0002] Omega-3 fatty acids in the phospholipid configuration have the advantages of high absorption and utilization by the human body and good stability. As a natural configuration of DHA and EPA, phospholipid is an active absorption method in the body, and the absorption rate is close to 100%, which is much higher than the passive absorption method of glyceride and ethyl ester, whose absorption rate is only 50% respectively. % and 20%. In addition, phospholipids themselves have high physiological activity, and phospholipid-type Omega-3 fatty acids are metabolized into phospholipids and Omega-3 fatty acids in the body, and the physiological functions of the two have a synergistic effect. Moreover, com...

Claims

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Application Information

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IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 彭永健许新德张莉华马金萍王胜南邵斌
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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