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Antimicrobial peptide and prokaryotic expression method and application thereof

A prokaryotic expression and antimicrobial peptide technology, applied in the field of genetic engineering, can solve the problems of low potency, limited sources of natural antibiotics, high cost of artificial synthesis, etc., and achieve the effect of simple method and obvious antibacterial effect in vitro

Active Publication Date: 2019-01-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, natural antibiotics also have disadvantages such as limited sources, low potency, and long polypeptide sequences leading to high artificial synthesis costs.

Method used

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  • Antimicrobial peptide and prokaryotic expression method and application thereof
  • Antimicrobial peptide and prokaryotic expression method and application thereof
  • Antimicrobial peptide and prokaryotic expression method and application thereof

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Embodiment Construction

[0032] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0033] One, the material and source that adopt in the specific embodiment of the present invention are as follows:

[0034] 1 strains and plasmids

[0035] DH5α Chemically Competent Cell (Beijing Qingke Xinye Biotechnology Co., Ltd.); BL21(DE3) Chemically Competent Cell (Shanghai Weidi Biotechnology Co., Ltd.); pMD19-T Vector (TaKaRa Company); pET-28a(+) vector, E .Coli and Staphylococcus aureus were preserved in our laboratory.

[0036] 2 reagents

[0037] High-fidelity enzyme PrimeSTAR HS DNA Polymerase, Taq enzyme, agarose gel electrophoresis 10×LoadingBuffer, DL1000DNA Marker, restriction endonuclease EcoRI and BamHI, plasmid ligation DNA LigationKit, nickel column His60Ni Gravity Columns used for protein purification are all from TaKaRa Company Products; AxyPrep Plasmid...

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Abstract

The invention discloses an antimicrobial peptide and a prokaryotic expression method and application thereof. The invention adopts prokaryotic expression vector to construct the recombinant vector ofthe encoding gene of the antibacterial peptide cecropin-BM, and prokaryotic expression of the antibacterial peptide cecropin-BM is achieved. The method is simple and easy, the purified recombinant antibacterial peptide has obvious antibacterial effect in vitro without any treatment.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an antibacterial peptide and its prokaryotic expression method and application. Background technique [0002] Antimicrobial peptide (antimicrobial peptide) is a small molecular polypeptide naturally present in almost all organisms, and is an important part of the natural immunity of organisms. Viruses also have a killing effect. Different from conventional antibiotics, antimicrobial peptides are not easy to cause bacterial drug resistance, and also have a killing effect on antibiotic-resistant strains, showing great potential in the field of new antibacterial drug development. However, natural antibiotics also have disadvantages such as limited sources, low potency, and long polypeptide sequences leading to high artificial synthesis costs. Therefore, it is of great significance to develop and prepare a new generation of antimicrobial peptides that can ov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/70
CPCC07K14/43586C12N15/70
Inventor 谭勋邵冯金应易恬
Owner ZHEJIANG UNIV
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