A preparation method of bran lactic acid fermented sheet rich in living bacteria

The technology of lactic acid fermentation and bran is applied in the field of preparation of bran lactic acid fermentation tablets, and can solve the problems of indigestion and absorption, low product utilization rate, rough taste and the like

Active Publication Date: 2021-09-14
合肥兴之诚信息科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to rough taste and difficult to digest and absorb when eaten directly, most of them can only be used in animal feed processing, auxiliary materials for wine making and other fields, and the product utilization rate is low.

Method used

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  • A preparation method of bran lactic acid fermented sheet rich in living bacteria
  • A preparation method of bran lactic acid fermented sheet rich in living bacteria
  • A preparation method of bran lactic acid fermented sheet rich in living bacteria

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Activation of lactic acid bacteria

[0036] Add 10 mL of MRS solid medium to two test tubes, sterilize at 121 °C for 20 min, place on an inclined plane to cool to 40 °C, and put 0.1 mL of glycerol-preserved LP strain bacterial liquid into one test tube on a sterile operating table. The other branch was inserted with 0.1 mL glycerol-preserved LF strain bacterial liquid, and cultured at 37°C for 24 hours. Then the MRS liquid medium was sterilized at 121°C for 20 minutes, and after cooling, the colonies of LP strain and LF strain that grew well on the slant medium were picked in an aseptic operating table, inoculated into the MRS liquid medium, and incubated at 37°C for 24 hours.

[0037] 2. High density culture of lactic acid bacteria

[0038] Inoculate the LP strain activated in step 1 with an inoculum amount of 1% in the sterilized LP growth medium and culture it at 37°C for 24 hours, collect the bacterial precipitate after centrifuging at 4000rpm for 10min, suspend...

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Abstract

The invention discloses a preparation method of bran lactic acid fermented sheet rich in living bacteria. The fermented agent prepared by mixing high-density cultivated Lactobacillus plantarum and Lactobacillus fermentum is inserted into a bran lactic acid fermentation substrate for lactic acid fermentation. fermentation. The present invention utilizes the rich carbohydrates in jujube to impart sour taste to food, improves the mouthfeel of bran, and promotes its digestion and absorption; mixing hawthorn and bran powder not only utilizes the dietary fiber in bran, but also utilizes the fruit in hawthorn Glue, vitamin C and other substances make the obtained product bran lactic acid fermentation sheet rich in dietary fiber. The bran lactic acid fermented sheet prepared by the present invention contains a large amount of lactic acid bacteria and rich lactic acid, acetic acid, succinic acid, and pectin, which can increase beneficial intestinal flora, promote the absorption of nutrients such as protein and calcium, and improve the human gastrointestinal tract function, enhance the body's immunity, make people energetic, promote body recovery, delay aging, and also have various health effects such as lowering cholesterol and blood sugar, and preventing gallstones.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to the high-density cultivation of two strains of lactic acid bacteria and the preparation of bran lactic acid fermented sheets rich in live bacteria. Background technique [0002] Lactic acid bacteria, composed of a group of bacteria with the same morphological, metabolic and physiological characteristics, can use sugars to produce large amounts of lactic acid. Lactic acid bacteria are closely related to human health, such as maintaining the ecological balance of intestinal flora, anti-hypertension, and enhancing human immunity. High-density culture technology is widely used in the field of microbial culture. It refers to the method of using certain culture technology and equipment to obtain a bacterial density higher than that of ordinary culture. High-density culture of lactic acid bacteria can increase the concentration of bacteria in the culture medium, reduce product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L29/00A23L29/30A23L33/00C12N1/20C12R1/25C12R1/225
CPCA23V2002/00A23L29/03A23L29/30A23L33/00C12N1/20A23V2200/32A23V2200/3262A23V2200/328A23V2200/3204A23V2200/324A23V2200/302
Inventor 张宝善白莉圆纪花颜统晶张晶余偲
Owner 合肥兴之诚信息科技有限公司
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