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Cultivation method of micro-shoot tip culture combined with heat treatment to obtain virus-free seedlings of Zengcheng Miriam chrysanthemum

A cultivation method and a technology of virus-free seedlings, which are applied in the field of cultivating Zengcheng Miriam chrysanthemum virus-free seedlings through micro-shoot tip culture combined with heat treatment, can solve the problems of slow propagation speed, low seedling cultivation, and low reproduction coefficient, and achieve fast propagation speed, Fast growth and high survival rate

Active Publication Date: 2020-02-21
广州田园牧歌农林股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is easy to obtain explants, the operation of tissue culture is simple, and the culture period is short, but without detoxification treatment, it is impossible to obtain seedlings with low virus infection rate, and the browning phenomenon in tissue culture is serious, which makes the explants gradually change. Brown and die, greatly reducing the survival rate of virus-free seedlings
[0007] At present, the detoxification culture technology for Compositae plants still has low detoxification rate and serious browning phenomenon in tissue culture, which greatly reduces the survival rate of detoxification seedlings, makes the reproduction speed slow, and the reproduction coefficient is low, making it impossible to carry out large-scale production. Large-scale industrial planting

Method used

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  • Cultivation method of micro-shoot tip culture combined with heat treatment to obtain virus-free seedlings of Zengcheng Miriam chrysanthemum
  • Cultivation method of micro-shoot tip culture combined with heat treatment to obtain virus-free seedlings of Zengcheng Miriam chrysanthemum
  • Cultivation method of micro-shoot tip culture combined with heat treatment to obtain virus-free seedlings of Zengcheng Miriam chrysanthemum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 A kind of cultivation method of micro shoot tip culture combined with heat treatment to obtain Zengcheng Michrysanthemum virus-free seedlings

[0046] The method for cultivating Zengcheng Michrysanthemum chrysanthemum virus-free seedlings in combination with heat treatment in described micro shoot tip culture, comprises the following steps:

[0047] (1) Breeding of superior strains

[0048] ①The first tissue culture seedling cultivation

[0049] Tissue culture seedlings were carried out on 9 excellent strains of the selected fine variety "Zengcheng Michrysanthemum", and 2500 seedlings were cultivated for each strain, a total of 22500 strains.

[0050] ②The first cultivation and planting test

[0051] The first cultivation and planting test was carried out on the tissue culture seedlings of 9 excellent strains. Each strain was planted on 1 mu, and 2,500 plants were planted per mu, and a total of 9 mu was planted.

[0052] ③ The second screening of excelle...

Embodiment 2

[0088] Embodiment 2 A kind of cultivating method of micro-shoot tip culture combined with heat treatment to obtain Zengcheng Miriam chrysanthemum virus-free seedlings

[0089] The method for cultivating Zengcheng Michrysanthemum chrysanthemum virus-free seedlings in combination with heat treatment in described micro shoot tip culture, comprises the following steps:

[0090] (1) Breeding of superior strains

[0091] Method is with embodiment 1.

[0092] (2) Explant pretreatment

[0093] Get the twigs in the 3 excellent individual plants that step (1) obtains as explants, the material used as explants puts into the plastic greenhouse 2 months before drawing materials and begins to stop leaching before drawing materials 10 days. Instead, use 1000 times of 70% thiophanate-methyl wettable powder aqueous solution every 3 days and wet the whole plant with potting soil for 3 consecutive times. When collecting materials, select normal and strong plants, remove leaves, and cut into 4...

Embodiment 3

[0117] Embodiment 3 A kind of cultivating method of micro shoot tip culture combined with heat treatment to obtain Zengcheng Michrysanthemum virus-free seedlings

[0118] The method for cultivating Zengcheng Michrysanthemum chrysanthemum virus-free seedlings in combination with heat treatment in described micro shoot tip culture, comprises the following steps:

[0119] (1) Breeding of superior strains

[0120] Method is with embodiment 1.

[0121] (2) Explant pretreatment

[0122] Get the twigs in the 3 excellent individual plants that step (1) obtains as explants, the material used as explants puts into the plastic greenhouse 2 months before drawing materials and begins to stop leaching before drawing materials 10 days. Instead, use 1000 times of 70% thiophanate-methyl wettable powder aqueous solution every 3 days and wet the whole plant with potting soil for 3 consecutive times. When collecting materials, select normal and strong plants, remove leaves, and cut into 4.0cm ...

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Abstract

The invention belongs to the technical field of the plant tissue culture, and particularly relates to a cultivating method for acquiring a Zengcheng honey chrysanthemum virus-free seedling through micro-stem tip culture combined with heat treatment. The provided cultivating method for acquiring the Zengcheng honey chrysanthemum virus-free seedling through the micro-stem tip culture combined with the heat treatment comprises the following steps: breeding a good strain, establishing a tissue culture sterile tissue culture vessel seedling, pre-eradicating viruses by heat treatment culture, eradicating viruses by the micro-stem tip culture, establishing a virus-free stem tip tissue culture sterile breeding system, detecting a virus-free stem tip tissue culture seedling virus and industrially growing the seedling through virus-free seedling tissue culture and the like. The provided method for acquiring the Zengcheng honey chrysanthemum virus-free seedling is capable of using the micro-stemtip tissue culture combined with heat treatment virus eradication, and cooperating with an improved culture medium, is high in virus-free seedling survival rate, rapid in breeding speed, and capable of industrially breeding the growing the seedlings through the large-scale tissue culture, and high in economic benefit.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a cultivation method for obtaining Zengcheng honeysuckle chrysanthemum virus-free seedlings through micro shoot tip cultivation combined with heat treatment. Background technique [0002] Chrysanthemum is the dry flower head of Asteraceae Chrysanthemum. It has the effects of dispelling wind and dissipating heat, clearing the liver and improving eyesight, and detoxifying sores. Modern pharmacological experiments have proved that chrysanthemum has the functions of improving cardiovascular function, anticancer, antibacterial, vasodilator, and antioxidation. The 2010 edition of "Chinese Pharmacopoeia-Part I" included chrysanthemum as a homologous plant of medicine and food, which has high economic value. In recent years, it has become an important undergrowth economic plant, which is widely planted in forests and hillsides, bringing huge economic and ecolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 巫宗华张华通罗晶晶张宏金
Owner 广州田园牧歌农林股份有限公司
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