A method based on proteomics to identify wool species in ancient woolen fragments

Abstract

The invention relates to the field of the culture relic detection, and discloses a method for identifying ancient wool fabric-pieces fur variety based on proteomics. The method specifically comprisesthe following steps: taking the same amounts of mixed ancient goat wool, sheep wool, camel hair fabric samples, extracting keratin by utilizing a metal salt method and a reduction method, respectivelyperforming SDS-PAGE gel electrophoresis to separate protein, and preparing carbon quantum dot solution as a staining solution by utilizing a reverse micro-emulsion method; performing enzymolysis on astrip, adopting the HPLC-MS / MS mass spectrography, comparing an amino acid sequence obtained through the mass spectrum detection with an amino acid sequence database to identify whether the ancient wool fabric is goat wool, sheep wool and camel hair. The detection method of the ancient wool fabric has the advantages of being clear in gel electrophoresis image, high in dissolving rate and extraction efficiency, and especially suitable for the identification of the fur variety of ancient wool fabric-pieces in different order-family species which are of early era and hard to be identified by thenaked eyes.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, and more specifically relates to a method for identifying wool types of ancient wool fabric fragments based on proteomics. Background technique [0002] In the Shang Dynasty four thousand years ago, the ethnic minorities living in the western region introduced a woolen fabric called "罽罽" to the Central Plains through offerings. Since then, woolen fabrics have appeared in the Central Plains. The ingredients of these wool fabrics include wool, camel hair, cow hair, rabbit hair and so on. [0003] A large number of wool products have been unearthed from early Chinese tombs. The quantity and the exquisite weaving are both amazing. The ethnic minorities in ancient my country used creative labor to produce wool fabrics with national characteristics. In the economic and cultural exchanges among the people of all ethnic groups, woolen fabrics have become a medium for friendly exchanges betwe...

AI Technical Summary

Problems solved by technology

After being unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, it is not difficult to imagine that in the long history, woolen fabrics buried in tombs or ruins have already lost their original appearance and degraded into Peptides or amino acids cannot be recognized by the naked eye, and the older the evidence, the harder it is to find, which brings great difficulties to the identification and protection of fur cultural relics

Moreover, for these ancient wool fabric fragments that are difficult to distinguish with the naked eye, it is even more difficult to distinguish the type of wool

[0005] At present, ordinary analysis and detection techniques, such as Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction analysis, etc., are often at a loss when encountering this kind of residue analysis. Most of the identification of ancient woolen fabrics at home and abroad are still stuck. In the analysis of fiber morphology, it is difficult to accurately identify different kinds of animal hairs, and there is nothing to do with different purpose animal hairs; therefore, it is very important to find a more scientific and convenient method to identify the hair types of ancient wool fabric fragments. necessary