A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof

A technology of Lactobacillus rhamnosus and culture method, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of salty taste, affecting application, high salinity of supernatant after centrifugation, etc., and achieve inorganic salt The effect of low content and stable strain

Active Publication Date: 2019-01-18
江西仁仁健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used medium for Lactobacillus rhamnosus (MRS) usually contains a relatively high concentration of inorganic salts, which will lead to high salinity of the bacteria, fermentation broth, and supernatant after centrifugation, and a salty taste, thus affecting its performance in agriculture and animal husbandry. Applications in important fields closely related to human life, such as industry, food and medicine

Method used

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  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof
  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof
  • A low-salt culture medium for Lactobacillus rhamnosus and a culture method thereof

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Embodiment 1

[0030] Embodiment 1 A kind of Lactobacillus rhamnosus substratum and culture method

[0031] (1) a culture medium for Lactobacillus rhamnosus, comprising: low-salt basal medium and low-salt optimization medium;

[0032] Formula and preparation of Lactobacillus rhamnosus low-salt basal medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomaltooligosaccharide 3g / L, L-apple Acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; weigh each component according to the formula ratio, mix, heat to dissolve, and sterilize at 115°C for 30min.

[0033] Formula and preparation of Lactobacillus rhamnosus low-salt optimized medium, formula: yeast peptone 22g / L, yeast powder 11g / L, glucose 32g / L, lactose 11g / L, isomaltooligosaccharide 3g / L, L-apple Acid 6g / L, citric acid 6g / L, manganese sulfate 0.06g / L, the balance is purified water, pH 6.8; weigh each component according to the formula ratio, mix, heat to dissolve, and sterilize a...

Embodiment 2

[0046] The single factor screening experiment of embodiment 2 low-salt basal medium components

[0047] At present, the most used culture medium for Lactobacillus rhamnosus is MRS medium. In order to achieve the purpose of low-salt culture and large-scale growth of Lactobacillus rhamnosus, it is necessary to find a low-salt medium suitable for the proliferation of Lactobacillus rhamnosus. It is applied to the large-scale industrial production of Lactobacillus rhamnosus freeze-dried powder as a food additive, and the low-salt improvement experiment of MRS medium is carried out.

[0048] Increase the content of nitrogen source and carbon source in the MRS medium, reduce the content of inorganic salts, conduct single factor experiments on the components of diammonium hydrogen citrate, dipotassium hydrogen phosphate, magnesium sulfate and manganese sulfate in the formula of MRS medium to investigate Effect of each component on the viable count of cultured Lactobacillus rhamnosus. ...

Embodiment 3

[0058] The single factor screening experiment of embodiment 3 low-salt optimized culture medium components

[0059] Select the bacterium liquid cultivated in the basal medium of Formula IV in Example 2, and inoculate the tertiary culture suspension into a 200L fermenter equipped with 120L low-salt optimized medium according to 5% inoculum size, and carry out the secondary culture of the bacteria. fermentation. Compared with the MRS medium, the low-salt optimized medium of Lactobacillus rhamnosus increases the content of nitrogen source and carbon source, reduces the type and content of inorganic salt, and removes Tween 80. The culture medium was prepared according to the following medium formulas of each group, and Lactobacillus rhamnosus was cultivated under the same culture conditions, and the number of viable bacteria in the fermentation broth and the conductivity of the supernatant after centrifuging the fermentation broth were detected.

[0060] MRS medium formula: yeast...

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Abstract

The invention discloses a low-salt culture medium of Lactobacillus rhamnosus comprising a basic culture medium and an optimized culture medium, wherein that basic culture medium comprises yeast peptone, yeast powder, glucose, lactose, isomaltooligosaccharide, L-Malic acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The optimized medium consistes of yeast peptone, yeast powder,glucose, lactose, isomaltooligosaccharide, L-Malic acid, citric acid, manganese sulfate, the balance of sterile water, pH 6.50-7.00. The culture medium is suitable for the propagation of Lactobacillusrhamnosus, and the number of viable bacteria in fermentation broth could reach more than 109 CFU / ml.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a low-salt culture medium and a culture method of Lactobacillus rhamnosus. Background technique [0002] Microbial culture medium (Medium) is an artificially prepared nutrient for the growth and maintenance of microorganisms. It mainly provides nutrients such as carbon sources and nitrogen sources required for the growth of microorganisms, and generally contains carbohydrates, nitrogenous substances, and inorganic salts (including trace elements) and water etc. Different media can add some growth factors according to actual needs. [0003] Studies have found that many modern chronic diseases, such as high blood pressure, kidney disease, upper respiratory tract infection, heart disease, etc., are positively correlated with excessive salt consumption. Low-salt diet has become a healthy way of life, so high salt is one of the key factors restricting the application of Lact...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20
Inventor 张春宇余萍王聪耀闵祥博
Owner 江西仁仁健康产业有限公司
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