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Method for extracting bonding polyphenol in carrot dietary fibers

A technology of dietary fiber and extraction method, which is applied in the field of health food production, can solve the problems of not being fully utilized, and achieve the effects of reducing oxidation, simple equipment, and simple steps

Inactive Publication Date: 2019-01-18
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many by-products of carrots, such as carrot skin and carrot residue, are often discarded so that they are not fully utilized, but studies have shown that there are high amounts of dietary fiber and polyphenols in discarded residue

Method used

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  • Method for extracting bonding polyphenol in carrot dietary fibers
  • Method for extracting bonding polyphenol in carrot dietary fibers
  • Method for extracting bonding polyphenol in carrot dietary fibers

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Embodiment

[0030] Materials and Reagents

[0031] Carrots were purchased from Tianhong Supermarket in Nanchang, Jiangxi. Before use, dry in an oven at 45°C, pulverize, and screen samples with a mesh number of 100 mesh for future use.

[0032] Hydrochloric acid, acetone, sodium hydroxide, and absolute ethanol are all of analytical grade.

[0033] High temperature resistant a-amylase (activity 200000u / g) and glucoamylase (activity 100000u / g) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., papain (activity 60000u / g) was purchased from Nanning Pangbo Bioengineering Co., Ltd.

[0034] 1. Experimental equipment

[0035] Pulverizer, FA1004B electronic balance, HWS-12 electric heating constant temperature water bath, drying oven, centrifuge, stirrer, spectrophotometer, pH meter.

[0036] 2. Experimental method

[0037] 2.1 Preparation of Carrot Dietary Fiber (CDF)

[0038] Take 10g of carrot powder (passed through a 100-mesh sieve) in a beaker, add a certain amount of...

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Abstract

The invention provides a method for extracting bonding polyphenol in carrot dietary fibers. The method comprises the following steps: (1) uniformly mixing 100-mesh sieved carrot powder with deionizedwater in a proportion of 1 to 8.5, sequentially adding high-temperature-resistant alpha-amylase, papain and saccharifying enzyme, respectively carrying out 30 minutes, so as to obtain the carrot dietary fibers; (2) adding the carrot dietary fibers into a brown spiral pipe filled with nitrogen, processing the carrot dietary fibers by virtue of a 2mol / l sodium hydroxide solution for 4 hours, adjusting the pH value of the solution to 1.5-2, carrying out extraction by virtue of an ethanol solution, filtering to obtain an alcohol extracting solution, carrying out vacuum concentration on the alcoholextracting solution, and carrying out freeze drying, so as to obtain bonding polyphenol. According to the method, by utilizing carrots as the raw material, bonding polyphenol in the dietary fibers isextracted by virtue of an optimal enzyme preparation process, and the structures of the dietary fibers are not damaged in the extraction process.

Description

technical field [0001] The invention relates to the technical field of health food production, in particular to a method for extracting bound polyphenols from carrot dietary fiber. Background technique [0002] Dietary fiber (DF) usually refers to the sum of edible plant components, carbohydrates and similar substances that cannot be digested and absorbed by the small intestine of the human body, but can be partially or fully fermented in the large intestine of the human body, generally composed of polysaccharides, oligosaccharides and woody prime composition. According to its solubility characteristics, it can be divided into water-soluble dietary fiber and water-insoluble dietary fiber. Although dietary fiber cannot be digested and absorbed by the human body, a large number of studies have shown that dietary fiber is related to many health benefits, including reducing the risk of cancer, cardiovascular disease, diabetes and some chronic diseases. The "seventh nutrient" a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L33/22
CPCA23V2002/00A23L33/105A23L33/22A23V2250/2132
Inventor 余强刘帅谢明勇聂少平万海生
Owner NANCHANG UNIV
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