Convenient and stable detection kit for homocysteine and detection method
A homocysteine, detection kit technology, applied in biological testing, measuring devices, material inspection products, etc., can solve sample processing, chromatographic condition sample detection and quantitative variation, cannot be popularized and applied, and expensive instruments, etc. problems, to achieve the effect of good matching, maintaining stability and sensitivity, and excellent surface performance
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Embodiment 1
[0046] A convenient and stable homocysteine detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:
[0047]
[0048]The components and concentrations included in the reagent R2 are:
[0049]
[0050] The components and concentrations included in the HCY liquid calibration solution are:
[0051] EDTA.2Na 10mmol / L
[0052] Krovin 750 45mmol / L
[0053] Homocysteine 28umol / L
Embodiment 2
[0055] A convenient and stable homocysteine detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:
[0056]
[0057] The components and concentrations included in the reagent R2 are:
[0058]
[0059] The components and concentrations included in the HCY liquid calibration solution are:
[0060] EDTA.2Na 10mmol / L
[0061] Krovin 750 45mmol / L
[0062] Homocysteine 28umol / L
[0063] When the cysteine detection kit described in Example 1-2 is used for detection, it is suitable for various automatic biochemical analyzers. Taking the Hitachi 7080 biochemical analyzer as an example, the rate method is used for determination. The specific steps are as follows: add 250ul Reagent R1 and 16ul sample, mix well, incubate at 37°C for 1-5min, add 25ul reagent R2, mix well, incubate at 37°C for 90s, continuously detect for 1-3min, calculate △A / min, the detection wavelength is The ...
Embodiment 3
[0093] A convenient and stable homocysteine detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:
[0094]
[0095] The components and concentrations included in the reagent R2 are:
[0096]
[0097] The components and concentrations included in the HCY liquid calibration solution are:
[0098] EDTA.2Na 60mmol / L
[0099] Krovin 750 50mmol / L
[0100] Homocysteine 28umol / L
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