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Convenient and stable detection kit for homocysteine and detection method

A homocysteine, detection kit technology, applied in biological testing, measuring devices, material inspection products, etc., can solve sample processing, chromatographic condition sample detection and quantitative variation, cannot be popularized and applied, and expensive instruments, etc. problems, to achieve the effect of good matching, maintaining stability and sensitivity, and excellent surface performance

Active Publication Date: 2019-01-15
QINGDAO HIGHTOP BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity and specificity are good, the instrument is expensive and cannot be widely used
High-performance liquid chromatography (HPLC) is a relatively mature and widely used method at present. The disadvantage is that there are many variations in sample processing, chromatographic conditions, sample detection and quantification, making it difficult to standardize
However, the attenuation of cystathionine β-lyase in the above two kits is still relatively fast, resulting in poor stability of the kits for cystathionine cycle enzyme method under the expiration date.

Method used

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  • Convenient and stable detection kit for homocysteine and detection method
  • Convenient and stable detection kit for homocysteine and detection method
  • Convenient and stable detection kit for homocysteine and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A convenient and stable homocysteine ​​detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:

[0047]

[0048]The components and concentrations included in the reagent R2 are:

[0049]

[0050] The components and concentrations included in the HCY liquid calibration solution are:

[0051] EDTA.2Na 10mmol / L

[0052] Krovin 750 45mmol / L

[0053] Homocysteine ​​28umol / L

Embodiment 2

[0055] A convenient and stable homocysteine ​​detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:

[0056]

[0057] The components and concentrations included in the reagent R2 are:

[0058]

[0059] The components and concentrations included in the HCY liquid calibration solution are:

[0060] EDTA.2Na 10mmol / L

[0061] Krovin 750 45mmol / L

[0062] Homocysteine ​​28umol / L

[0063] When the cysteine ​​detection kit described in Example 1-2 is used for detection, it is suitable for various automatic biochemical analyzers. Taking the Hitachi 7080 biochemical analyzer as an example, the rate method is used for determination. The specific steps are as follows: add 250ul Reagent R1 and 16ul sample, mix well, incubate at 37°C for 1-5min, add 25ul reagent R2, mix well, incubate at 37°C for 90s, continuously detect for 1-3min, calculate △A / min, the detection wavelength is The ...

Embodiment 3

[0093] A convenient and stable homocysteine ​​detection kit, including reagent R1, reagent R2 and HCY liquid calibration solution, the components and concentrations contained in the reagent R1 are:

[0094]

[0095] The components and concentrations included in the reagent R2 are:

[0096]

[0097] The components and concentrations included in the HCY liquid calibration solution are:

[0098] EDTA.2Na 60mmol / L

[0099] Krovin 750 50mmol / L

[0100] Homocysteine ​​28umol / L

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Abstract

The invention provides a convenient and stable detection kit for homocysteine and a detection method. The kit comprises a reagent R1, a reagent R2, and a HCY liquid calibration solution. The reagent R1 contains a buffer, a reducing agent, a serine, a coenzyme preservative, and a surfactant. The reagent R2 contains a buffer, a surfactant, a lactate dehydrogenase, a stabilizer, an antioxidant, a preservative, a cystathionine beta-synthase, and a cystathionine beta-lyase. The HCY liquid calibration solution contains a stabilizer, a preservative, and homocysteine. The antioxidant is added into thekit, so that the attenuation of the enzyme under various interference factors is inhibited, and the stability of the kit in the special environment and the validity period is effectively improved.

Description

technical field [0001] The invention belongs to the field of medical in vitro diagnostic reagents, and in particular relates to a convenient and stable homocysteine ​​detection kit and detection method. Background technique [0002] Blood homocysteine ​​(HCY) is a risk factor for stroke and other cardiovascular and cerebrovascular diseases. Its level is significantly increased, and it can be diagnosed as "hyperhomocysteinemia", especially if accompanied by a history of hypertension. Many scholars believe that elevated blood homocysteine ​​has become an independent risk factor for atherosclerosis, and can cause a variety of diseases, because it can affect the synthesis and function of reduced glutathione, and affect normal liver function. The specificity and sensitivity of this indicator for the diagnosis of chronic liver disease are better than those of conventional detection; another new concept is that essential hypertension accompanied by elevated plasma HCY (>10umol / L...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6815
Inventor 杨帆唐玉蓉高洪元李滨雁宋金玲刘美雪吕萌萌
Owner QINGDAO HIGHTOP BIOTECH
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