Multi-beam multiphoton microscopic imaging device

Abstract

The invention provides a multi-beam multiphoton microscopic imaging device, comprising a light source module, a beam splitter, a resonance-galvanometer scanning head, a focus module and an imaging module. The light source module is used for generating a laser which can be used for multiphoton excitation. The beam splitter is used for generating a plurality of laser beams in a straight line of equiangular pitch. The scanning direction of the resonance scanning head and the galvanometer scanning head are perpendicular to each other, and the arrangement direction of the plurality of laser beams is consistent with the scanning direction of the galvanometer scanning head. The focus module is used for converting the laser emitted by the resonance-galvanometer scanning head into a focus spot of equiangular pitch and irradiating to the sample so as to excite a fluorescent or multiphoton high-order harmonic signal. The imaging module is for collecting fluorescent or multiphoton high-order harmonic signal for imaging. The multi-beam multiphoton microscopic imaging device provided by the invention adopts a plurality of laser beams located in a straight line, and the arrangement direction of the plurality of laser beams is consistent with the scanning direction of the galvanometer scanning head, thereby the plurality of laser beams scanning the sample simultaneously, which improves the imaging speed.

Description

technical field [0001] The present invention relates to the field of optical technology, more specifically, to a multi-beam multi-photon microscopic imaging device. Background technique [0002] With the development of science and technology, optical microscopic imaging technology has become an important technology and measurement method in biology, medicine and related interdisciplinary research. Due to the characteristics of non-invasive, non-invasive, and fast acquisition, optical microscopy imaging technology is very suitable for imaging of living cells, biological tissues and non-biological systems, and has advantages that cannot be replaced by other technologies. Therefore, in recent years, optical microscopic imaging technology has always been the frontier and hot spot of technological development, and has continuously achieved new developments and breakthroughs. Its development trend is higher spatial resolution, faster speed, and dynamic biological information acqui...

AI Technical Summary

Problems solved by technology

[0003] A variety of focused illumination scanning imaging methods are provided in the prior art, but the imaging time resolution is low, and it is impossible to observe the biochemical dynamics in living cells and tissues at the millisecond and sub-millisecond levels, such as neuron activity and calcium signal. Fluctuation and excitation, molecular mechanism of neurotransmitter secretion, etc.

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