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Bacterial wilt resistance biocontrol strain and an application thereof

A biocontrol strain and bacterial wilt resistance technology, applied in application, bacteria, biocide and other directions, can solve problems such as difficulty in large-scale production, promotion and use, and unsatisfactory control effects, and achieve improved quality, environmental friendliness, and stable passage. Effect

Active Publication Date: 2019-01-15
SHENYANG RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This invention provides a strain of Streptomyces GIM.116, whose metabolite has antibacterial activity, and its antibacterial effect only has good inhibitory effect on Escherichia coli and Staphylococcus aureus with multi-drug resistance, but when its metabolite is used , needs to be extracted with ethyl acetate organic solvent, which is not easy for large-scale production and large-scale farmland promotion and use
[0006] CN102146350 B discloses the biocontrol bacterial strain 3YW8 of preventing and treating ginger bacterial wilt, which is a pseudo-odour-like fungus, belonging to the bacterial species, and inhibits the growth of pathogenic bacteria through competition for the prevention and treatment of ginger bacterial wilt. The control effect is 59.68%, and the control effect is not very ideal. Therefore, there is an urgent need for highly effective biocontrol inhibitors for this type of pathogens for different crop objects

Method used

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  • Bacterial wilt resistance biocontrol strain and an application thereof
  • Bacterial wilt resistance biocontrol strain and an application thereof
  • Bacterial wilt resistance biocontrol strain and an application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: Screening and isolation of biocontrol bacteria SY-SF-03

[0025] Collect 10g of soil from the woodland in Northeast China, add it to a triangular flask filled with 100mL sterile water and glass beads, shake it on a shaker at 150r / min for 30min, let it stand for 1h after shaking, take 10mL of the supernatant, and add it to 90mL sterile water , after mixing evenly, serially dilute 10 -2 、10 -3 、10 -4 、10 -5 Take 10 μL of the diluted solution and apply it on a sterile Gaussian No. 1 solid medium plate, apply three parallels for each gradient, and culture it in a constant temperature incubator at 28°C for 3-7 days, pick a single colony in the medium and carry out Streak and purify the plate to obtain a single colony of bacteria SY-SF-03, a single colony of bacteria figure 1 shown.

Embodiment 2

[0026] Embodiment 2: the identification of biocontrol bacteria SY-SF-03

[0027] Strain SY-SF-03 was cultured on Gaoshi No. 1 solid medium. The colony is round, with a raised surface and off-white edge. The mycelium in the base is light gray at first, and the mycelium has no transverse septum. After producing red pigment, it turns red. The aerial hyphae are white, and the color after spore production is dark gray. The spores are oval or spherical under a microscope (Figure 2). The 16SrRNA sequence of the strain SY-SF-03 was extracted and sequenced by Suzhou Jinweizhi Biotechnology Co., Ltd. The sequencing results were compared with the GenBank database sequence. The results showed that the strain SY-SF-03 belonged to Streptomyces venezuela in the genus Streptomyces (Streptomyces) (Streptomyces venezuelae), which has been preserved in the China Center for Type Culture Collection on May 14, 2018, the preservation address: Wuhan University, No. 299, Bayi Road, Wuchang District, W...

Embodiment 3

[0030] Embodiment 3: the antibacterial effect of biocontrol bacteria SY-SF-03 to R. solanacearum

[0031] Plate confrontation method was used to investigate the inhibitory effect of strain SY-SF-03 on R. solanacearum: inoculate the isolated actinomycete Streptomyces venezuelae in the center of the TSB plate and culture it in an incubator at 28°C for 3 days. , with a sterile throat sprayer at a concentration of 2 x 10 8 The cfu / mL suspension of R. solanacearum was sprayed onto the TSB plate, and after standing for 20 minutes, the plate was turned upside down and incubated in a 30°C incubator. After 24 hours, the diameter of the inhibition zone was observed and measured. The diameter of the inhibition zone on the observation plate can reach 42mm, such as image 3 shown.

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Abstract

The invention relates to a bacterial wilt control technology, in particular to a bacterial wilt resistance biocontrol strain and an application thereof. Biocontrol strain SY-SF-Streptomyces venezuelaebelonging to the genus Streptomyces (Streptomyces) is isolated from Venezuela on 14 May 2018, Deposited in China Typical Culture Collection Center, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, China. Deposited No.: CCTCC M2018265. A Streptomyces venezuelanensis strain resistant to bacterial wilt is obtain through screening in northeast woodland and identify as Streptomyces venezuelanensis, which can antagonize bacterial wilt pathogen Ralstonia solanacearum, and that fermentation metabolite thereof can inhibit the growth and multiplication of pathogen and preventthe occurrence of bacterial wilt.

Description

technical field [0001] The invention relates to the prevention and control technology of bacterial wilt, in particular to a bacterial wilt-resistant biocontrol strain and its application. Background technique [0002] Bacterial wilt is a devastating soil-borne disease caused by Ralstonia solanacearum, which can harm more than 300 species of plants in 44 families and is widely distributed in tropical, subtropical and temperate regions. , the hosts with severe damage and wide distribution mainly include potato, tomato, tobacco, eggplant, pepper, peanut, ginger, banana, ganru, hemp and so on. Bacterial wilt is one of the most harmful, widely distributed, and most costly plant diseases. In recent years, with global climate change and rising temperatures, the harm of bacterial wilt has increased year by year. major obstacle. [0003] At present, the control methods for bacterial wilt are mainly to improve the resistance of plants, realize tillage control, chemical control and b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/61
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 王加友王芳王远杨德玉赵彭年魏婷婷王洪庆
Owner SHENYANG RES INST OF CHEM IND
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