Red-skin hard-seed pomegranate tissue culture propagation system

Abstract

The invention discloses a red-skin hard-seed pomegranate tissue culture system. The culture system involves explant selection, differentiation seedling induction, differentiation seedling proliferation culture, differentiation seedling rooting culture and tissue culture seedling field transplanting. The medium includes a primary medium, a proliferation medium and a rooting medium. The invention also relates to a red-skin hard-seed pomegranate tissue culture induction method. The three mediums are used together. By adding PVP and weak light culture, browning rate is effectively reduced; by regulating the medium and coconut juice, yellowing and withering and stem tip necrosis are improved. The culture period of tissue cultured seedlings can be shortened by the preparation of the primary medium, the proliferation medium and the rooting medium. It shows through research results that the phenomenon of withering of pomegranate proliferation buds is related to excessive content of ammonium salt. In addition, the addition of GA3 in the proliferation medium can effectively prevent the production of vitrified seedlings and improve the quality of differentiation of test-tube seedlings; and the addition of AgNO3 in the proliferation medium can effectively increase the proliferation coefficient of pomegranate.

Description

technical field [0001] The invention belongs to the field of fruit tree cultivation agricultural biology technology, and mainly relates to a pomegranate tissue culture method and culture medium. Background technique [0002] Pomegranate (Punica granatum L.) belongs to Lythraceae (Lythraceae) pomegranate (Punica L.) deciduous shrubs or small trees, is an ancient fruit tree species. It is native to Central Asia such as Iran, Afghanistan, and the Caucasus, and has a cultivation history of more than 3,000 years. It was introduced to my country by Zhang Qian in the Western Regions from 138 to 125 BC. It is one of the earliest fruit tree species introduced in my country. Studies have shown that its fruit is rich in bioactive substances such as phenolic compounds, and has many nutritional values ​​and health care functions. Comprehensive research has deepened day by day, and as a new fruit tree, the industrial scale of the main producing countries has increased rapidly. At the sam...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for tissue culture and propagation of red-skinned hard-seeded pomegranate, which solves the technical difficulties in the selection of hard-seeded pomegranate explants, explant disinfection methods, inoculation, cultivation in a culture room, hardening seedlings, etc., and at the same time provides a Hard-seed pomegranate medium, solves the problem of rapid mass propagation of pomegranate seedlings, and makes up for the problems of high explant contamination rate, low differentiation rate, low proliferation coefficient, and low transplanting survival rate of traditional technologies

Images