A kind of magnetic particle buffer for prolactin assay kit and its application

Abstract

The invention discloses a magnetic particle buffer used in a prolactin assay kit and an application thereof. The magnetic particle buffer is composed of a basic buffer, an antifoaming agent and citrate; wherein, in 1L of the basic buffering solution, the amount of the antifoaming agent is added at a final concentration of 0.001% to 0.1% v / v, lemon The amount of salt added is added at a final concentration of 0.06-0.5 mol / L. The magnetic particles using the buffer solution not only have good dispersibility under natural gravity conditions, are not easy to foam during the mixing process, but also have excellent stability, which effectively improves the stability of the prolactin antibody on the magnetic particles.

Description

technical field [0001] The invention relates to the field of chemiluminescent immunoassay, in particular to a magnetic particle buffer used in a prolactin assay kit and an application thereof. Background technique [0002] Prolactin (Human Prolactin, Lactogenic Hormone, PRL) is a protein hormone secreted by the pituitary gland. Structurally, it belongs to the same protein family as growth hormone (hGH) and placental lactogen (hPL). PRL contains 199 amino acids, and it is similar to hGH and hPL Amino acid sequences have 26% and 27% homology, respectively. The determination of PRL can be used as an important index to evaluate the hypothalamus-pituitary function, especially for the hyperprolactinemia caused by various factors such as pituitary prolactinoma, and the diagnosis of hypothalamus diseases; It is of great significance to diagnose and differentiate the etiology of menstrual abnormalities and infertility. [0003] At present, the mainstream method for measuring prolac...

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Problems solved by technology

[0004] However, because the density of immunomagnetic particles is much higher than that of water, precipitation and surface adhesion of containers are often prone to occur in aqueous phase storage reagents.

During the detection process, if the immunomagnetic particles are unevenly dispersed in the storage system, foams are likely to be generated after mixing, which may cause obvious deviations in the subsequent measurement results; therefore, it is necessary to add an auxiliary agent to the magnetic particle buffer, To solve the problems of mutual aggregation, sedimentation and easy foaming of magnetic particles

However, the addition of auxiliary agents for sedimentation and foaming often affects the stability of magnetic particles coated with antibodies; in order to further improve the stability of antibodies, it is necessary to add new auxiliary agents, which makes the magnetic particle buffer components complex and expensive. high, increased risk

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