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Method for analyzing and detecting L-phenylalanine based on liquid crystal sensor

A detection method, the technology of phenylalanine, applied in the analysis of materials, material analysis through optical means, instruments, etc., can solve the problems of long test cycle, complicated instruments, labor-intensive, etc., and achieve short sample processing time and high detection method The effect of simplicity and low reagent consumption

Inactive Publication Date: 2019-01-11
SHANDONG UNIV
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  • Abstract
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Problems solved by technology

The electrochemical method is an analysis method of L-phenylalanine established on the basis of the electrical response signal, and the selectivity is slightly poor; the high-performance liquid chromatography is to inject the sample to be tested, and after the components in the column are separated, they enter the detection sequence in turn. The detection method is time-consuming; the chemiluminescence method requires molecular labels or nanoprobes; these methods have a long test cycle, labor-intensive, complex instruments, and are not suitable for rapid, sensitive and selective detection of L-phenylalanine

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  • Method for analyzing and detecting L-phenylalanine based on liquid crystal sensor
  • Method for analyzing and detecting L-phenylalanine based on liquid crystal sensor
  • Method for analyzing and detecting L-phenylalanine based on liquid crystal sensor

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Embodiment 1

[0051] The preparation of embodiment 1 liquid crystal sensor

[0052] The preparation method of the liquid crystal sensor is as follows:

[0053] i. With the glass slide as the substrate of the liquid crystal sensor, soak the glass slide in the "piranha" washing solution, soak for 50 minutes at 75°C, then wash with water, absolute ethanol, and methanol for 3 times respectively, and blow dry with nitrogen , dried overnight at 115°C; place the overnight dried glass slide in a solution of octadecyltrichlorosilane-n-heptane with a concentration of 5mM for 30min, rinse with dichloromethane, and blow dry with nitrogen to obtain the treated rear liquid crystal substrate;

[0054] ii. Copper mesh is laid on the upper surface of the treated liquid crystal substrate obtained in step i, and 4-cyano-4'-pentylbiphenyl is added dropwise on the liquid crystal substrate covered with copper mesh to prepare a liquid crystal sensor.

[0055] Among them, the "piranha" lotion is prepared by usin...

Embodiment 2

[0057] Example 2 Detection of Alkaline Phosphatase of L-Phenylalanine and Determination of Substrate Dodecyl Phosphate Sodium Salt Concentration

[0058] Dissolve dodecyl phosphate sodium salt in TBS buffer solution by ultrasonic, the ultrasonic dissolution time is 35min, and prepare dodecyl phosphate with concentrations of 0, 0.025mM, 0.05mM, 0.1mM, 0.2mM and 0.4mM respectively Esters sodium salt solution, the above-mentioned dodecyl phosphate sodium salt solution of different concentrations was respectively dropped on the upper surface of the liquid crystal sensor prepared in Example 1, and the dropping volume was 40 μ L, and observed using a polarizing microscope, the results were as follows figure 2 As shown, when the concentration of dodecyl phosphate sodium salt solution<0.2mM, the liquid crystal presents a bright appearance; when the concentration of dodecyl phosphate sodium salt solution is ≥0.2mM, the liquid crystal presents a dark appearance .

[0059] The dodecyl ...

Embodiment 3

[0061] Embodiment 3 Analysis and detection of L-phenylalanine standard substance based on liquid crystal sensor

[0062] The standard L-phenylalanine (purchased from Bailingwei Technology Co., Ltd., product number: 162943) was ultrasonically dissolved in TBS buffer, and the time of ultrasonic dissolution was 35min. / mL, 10ng / mL, 1ng / mL, 0.1ng / mL, 0.01ng / mL, 0.001ng / mL, 0.0001ng / mL L-phenylalanine standard solution; Add alkaline phosphatase solution to the acid standard solution, mix well, the volume ratio of alkaline phosphatase solution and L-phenylalanine standard solution is 1:1, the concentration of alkaline phosphatase in the mixed solution 10 μg / mL, react at 37°C for 50 minutes to obtain the enzyme reaction solution, drop the above enzyme reaction solution on the upper surface of the liquid crystal sensor modified with 40 μL 0.4 mM dodecyl phosphate sodium salt solution, the dropping volume is 40 μL, Observed using a polarizing microscope, the results are as follows F...

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Abstract

The invention relates to a method for analyzing and detecting L-phenylalanine based on a liquid crystal sensor. The method comprises the following steps: (1) diluting a sample to be tested in a TBS buffer solution to prepare a solution to be tested; (2) adding an alkaline phosphatase solution into the solution to be tested to prepare an enzyme reaction solution after a reaction; and (3) dripping the enzyme reaction solution onto the liquid crystal sensor modified by a sodium lauryl phosphate solution, and performing observation by using a polarizing microscope. The method for analyzing and detecting the L-phenylalanine in the urine of a patient with phenylketonuria based on the liquid crystal sensor provided by the invention has the advantages of low detection limit, easiness to obtain thedetection instrument, simple, rapid and sensitive detection method, less reagent consumption, short sample processing time and the like, the problems that the existing detection method is complicatedand high in cost are solved.

Description

technical field [0001] The invention relates to an analysis and detection method of L-phenylalanine based on a liquid crystal sensor, and belongs to the technical field of amino acid detection by enzyme inhibition method. Background technique [0002] As one of the eight essential amino acids for humans, L-phenylalanine is an important biomarker in clinical diagnosis and has an important impact on human health, such as the diagnosis and treatment of phenylketonuria. Therefore, L-phenylalanine The research and detection of alanine has been widely concerned by people. [0003] Reported methods for detecting L-phenylalanine, such as electrochemical method, high performance liquid chromatography, chemiluminescence (see Y.F.Hu, Z.H.Zhang, H.B.Zhang, L.J.Luo, S.Z.Yao, Talanta, 84, 2011, 305-313; T. Terrlink, P.A. Van Leeuwen, & A. Houdijk, Clinical chemistry, 40, 1994, 245-249; M. Kamruzzaman, A.-M. Alam, K.M. Kim, S.H. Lee, Y.H. Kim, G .-M. Kim, T.D. Dang, Food Chem. 135, 2012,...

Claims

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Application Information

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IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 于丽周乐乐亓鲁滨马慧鹿洁
Owner SHANDONG UNIV
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