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Compound probiotic deodorant, preparation method thereof and application thereof

A compound probiotic and deodorant technology, which is applied in the field of deodorization in farms, can solve the problems of low strain synergy, short storage time, poor stability, etc., achieve high safety and improve deodorization effect

Pending Publication Date: 2019-01-11
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, most of the existing biological deodorants are made by fermenting each probiotic separately first, and then mixing and compounding them. The synergy between the strains is low, the stability is poor, and the storage time is short, which is difficult to meet the actual needs.

Method used

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  • Compound probiotic deodorant, preparation method thereof and application thereof
  • Compound probiotic deodorant, preparation method thereof and application thereof
  • Compound probiotic deodorant, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of preparation process of compound probiotic deodorant is as follows:

[0033] 1. Preparation of seed solution

[0034] (1) Yeast: Inoculate Hansenula anomalies and Saccharomyces cerevisiae into YPD liquid medium, culture at 30-35°C, 150-200r / min, for 20-24h, divide into shake flasks, and prepare 1.5L mixed bacteria liquid, the number of viable bacteria ≥ 5.0×10 8 CFU / mL.

[0035] (2) Bacillus: Inoculate Bacillus subtilis and Bacillus coagulans in LB liquid medium, culture at 35-37°C, 150-200r / min, for 20-24h, prepare 1.5L mixed bacterial solution, the number of viable bacteria is ≥5.0 ×10 8 CFU / mL.

[0036] (3) Lactic acid bacteria: Inoculate Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecalis, and Enterococcus faecium into MRS liquid medium, culture at 35-37°C for 30-36 hours, prepare 1.5L of mixed bacterial solution, live Bacterial count≥5.0×10 8 CFU / mL.

[0037] 2. Fermentation tank expansion

[0038] (1) Expansion culture medium...

Embodiment 2

[0065] A compound probiotic deodorant for deodorizing farms, the preparation method of which comprises the following steps:

[0066] 1) Prepare yeast mixed bacteria liquid, bacillus mixed bacteria liquid and lactic acid bacteria mixed bacteria liquid respectively:

[0067] In the yeast mixture liquid, yeasts include Hansenula anomalies and Saccharomyces cerevisiae, and the number of viable bacteria is ≥5.0×10 8 CFU / mL; in the mixed bacillus liquid, bacillus includes bacillus subtilis and bacillus coagulans, the number of viable bacteria is ≥5.0×10 8 CFU / mL; in the mixed lactic acid bacteria solution, lactic acid bacteria include Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecalis and Enterococcus faecium, the number of viable bacteria is ≥5.0×10 8 CFU / mL;

[0068] 2) Prepare expansion culture medium, which includes the following components and parts by weight: 30 parts of cornstarch, 30 parts of soybean meal powder, 8 parts of corn steep liquor powder, ...

Embodiment 3

[0072] A compound probiotic deodorant for deodorizing farms, the preparation method of which comprises the following steps:

[0073] 1) Prepare yeast mixed bacteria liquid, bacillus mixed bacteria liquid and lactic acid bacteria mixed bacteria liquid respectively:

[0074] In the yeast mixture liquid, yeasts include Hansenula anomalies and Saccharomyces cerevisiae, and the number of viable bacteria is ≥5.0×10 8 CFU / mL; in the mixed bacillus liquid, bacillus includes bacillus subtilis and bacillus coagulans, the number of viable bacteria is ≥5.0×10 8 CFU / mL; in the mixed lactic acid bacteria solution, lactic acid bacteria include Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecalis and Enterococcus faecium, the number of viable bacteria is ≥5.0×10 8 CFU / mL;

[0075] 2) Prepare expansion culture medium, which includes the following components and parts by weight: 40 parts of cornstarch, 20 parts of soybean meal powder, 10 parts of corn steep liquor powder,...

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Abstract

The invention relates to a composite probiotic deodorant, a preparation method and an application thereof. The preparation method comprises the following steps: 1) respectively preparing yeast mixed bacterial solution, bacillus mixed bacterial solution and lactic acid bacterial mixed bacterial solution; 2) adding that yeast mixed bacterial liquid and the bacillus mix bacterial liquid into a spreading culture medium for aerobic fermentation; 3) adding that mixed lactic acid bacteria liquid into the spread culture medium in the step 2) and continuing anaerobic fermentation to obtain a compositeprobiotic deodorant; Deodorant is used to deodorize farms. As compare with that prior art, the deodorant of the invention is prepared by mix and fermenting a plurality of probiotics, the synergy amongthe strains is good, the preservation time of the live bacteria is prolonged, the deodorant function is diversified, and the deodorant is convenient to use.

Description

technical field [0001] The invention belongs to the technical field of farm deodorization, and relates to a compound probiotic deodorant and its preparation method and application. Background technique [0002] In recent years, my country's livestock and poultry breeding industry has developed rapidly, and large-scale farming has become more and more common. Along with this, the environmental pollution of livestock and poultry breeding has become increasingly serious. Among them, the odor pollution produced by livestock and poultry manure is one of the important environmental pollution of livestock and poultry farms. Livestock manure contains harmful gases such as ammonia and hydrogen sulfide. If it cannot be treated in time, it will not only affect the growth of livestock and poultry in the farm, but also pose a threat to the surrounding environment and human health. [0003] At present, deodorization methods for farms can generally be divided into three categories: physic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/18B01D53/84C12R1/78C12R1/865C12R1/125C12R1/07C12R1/23C12R1/25C12R1/01
CPCB01D53/84B01D2251/95B01D2258/0266C12N1/16C12N1/18C12N1/20Y02A50/20
Inventor 赖水明张诗覃智斌肖俊峰吴有林傅心锋
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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