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A kind of functionalized nano cellulose hydrogel and preparation method thereof

A technology of nanocellulose and oxidized cellulose, applied in prosthesis, medical science, etc., can solve the problems of inability of cells to grow and migrate, harsh conditions, unfavorable cell mixing, etc., and achieve good biocompatibility, high water content, biological good compatibility

Active Publication Date: 2021-07-30
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods either need to introduce biotoxic chemical cross-linking agents, or need to modify the cellulose, or the conditions are relatively harsh (such as heating or freezing), which is not conducive to mixing directly with cells, and usually the prepared hydrogel With a nanopore structure, cells can only grow on the surface of the gel, and cells cannot grow and migrate inside the gel

Method used

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  • A kind of functionalized nano cellulose hydrogel and preparation method thereof
  • A kind of functionalized nano cellulose hydrogel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A functionalized nanocellulose hydrogel for 3D bioprinting, the preparation method is as follows:

[0034] 1) Add 0.01g TEMPO and 0.1g NaBr into 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g of cotton linters (length 2-3mm ) and 1gNaClO, adjust the pH of the system to 8 by adding 0.1mol / L NaOH solution dropwise, after reacting at 10°C for 3h, continue to add 1gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC). 2 Perform acidification to oxidize incompletely oxidized aldehyde groups into carboxylate groups, which is conducive to better dispersion of nanofibers;

[0035] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0036]3) Preparation of nanocellulose gel: T...

Embodiment 2

[0039] A functionalized nanocellulose hydrogel for 3D bioprinting, the preparation method is as follows:

[0040] 1) Add 0.1g TEMPO and 0.1g NaBr to 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g cotton linters (length 2-3mm) to the above system and 1gNaClO, by dropping 0.1mol / L NaOH solution to adjust the pH value of the system to 8, after reacting at 10°C for 4h, continue to add 1gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC);

[0041] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0042] 3) Preparation of nanocellulose gel: Concentrate the above TOCN dispersion to a concentration of 0.2%, heat it in a sterilized pot at 121°C for 30 minutes, and then mix the 0.2...

Embodiment 3

[0045] A 3D bioprinted nanocellulose gel can be prepared by the following method:

[0046] 1) Add 0.01g TEMPO and 0.1g NaBr to 100mL deionized water, stir magnetically at 10°C and 300r / min for 10min to completely dissolve TEMPO and NaBr, then add 1g cotton linters (length 2-3mm) to the above system and 0.5gNaClO, adjust the pH value of the system to 8 by adding 0.1mol / L NaOH solution dropwise, after reacting at 10°C for 3h, continue to add 0.5gNaClO 2 Stir and react at room temperature for 1 day, filter the oxidized cellulose pulp, wash with deionized water for more than 3 times, and dry to obtain TEMPO oxidized cellulose (TOC);

[0047] 2) Prepare TOCN dispersion: add 0.1g TOC to 99.9g distilled water and mechanically stir at 1600r / min for 10min to obtain 0.1wt% TOCN dispersion;

[0048] 3) Preparation of nanocellulose gel: Concentrate the above TOCN dispersion to 0.5%, heat it in a sterilized pot at 121°C for 30 minutes, and then mix the nanocellulose solution with a concen...

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PUM

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Abstract

The invention relates to a functionalized nano-cellulose hydrogel and a preparation method thereof. The interior of the hydrogel presents a uniform porous network structure with a pore diameter of 30 to 100 microns, and is prepared by dispersing TEMPO oxidized cellulose in water The nanocellulose dispersion is mixed with cells to make bio-ink, and then printed into ion cross-linking agent by 3D bio-printing technology to prepare, and its shape can be designed according to needs. The present invention uses renewable resource cellulose as a raw material to prepare a functionalized nanocellulose hydrogel with excellent mechanical properties, good biocompatibility and suitable for bioprinting, which is used as a nanocellulose-based tissue engineering material, specifically for bone , cartilage, skin, blood vessels, liver, heart and other tissues, which broaden the application range of cellulose and provide a new way for the application research of cellulose in the field of biomedicine.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a functionalized nano-cellulose hydrogel and a preparation method thereof. Background technique [0002] 3D bioprinting technology is a method of using computer-assisted transfer process to simultaneously write living cells and biomaterials in a predetermined layer-by-layer stacking manner to prepare bioengineered tissues. 3D bioprinting technology uses water dispersion and cells as the main raw materials, and the main form of the product is hydrogel. There are many requirements for hydrogel, such as good biocompatibility, and no clogging of needles, easy to print, and at the same time during the printing process The shear force has little damage to cells, so there are not many hydrogels that can be used for 3D bioprinting. [0003] As a polysaccharide with a crystalline structure, cellulose is the most abundant natural polymer on the earth. The cellulose hydrogel processed fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/00C08J3/075C08J3/24C08L1/04A61L27/20A61L27/38A61L27/56A61L27/52A61L27/50
CPCA61L27/20A61L27/3834A61L27/50A61L27/52A61L27/56C08J3/075C08J3/24C08J9/00C08J2301/04C08L1/04
Inventor 石竹群徐海宇杨全岭刘妍熊传溪
Owner WUHAN UNIV OF TECH
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